miRNA: 3'- -UAGUUCGC-GUGGUCUGG--CAGGcu -5'
miRNA: 3'- uaGUUCGCGUGGUCUGGCa-------GGCU- -5'
miRNA: 3'- uaGUUCGCGUGGUCUGGcAGGcu -5'
miRNA: 3'- -UAGUUCGCGUGGUCUGGCAGGCU- -5'
miRNA: 3'- -UAGUUCGCGUGGUCUGGCAGGCU- -5'
Results 21 - 25 of 25 are showing below:
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| Acc. No. | Name | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| MIMAT0003444 | rlcv-miR-rL1-14-5p | -52.6 | NC_006146.1 | + | 50304 | 0.74 | 0.584518 |
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Target: 5'- uGUCAGGCGcCGCCGGGCCuuGUCCa- -3' miRNA: 3'- -UAGUUCGC-GUGGUCUGG--CAGGcu -5' |
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| MIMAT0003444 | rlcv-miR-rL1-14-5p | -52.6 | NC_006146.1 | - | 138010 | 0.76 | 0.470476 |
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Target: 5'- -cCAGGUGCACCGGACCGgcggacccCCGAa -3' miRNA: 3'- uaGUUCGCGUGGUCUGGCa-------GGCU- -5' |
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| MIMAT0003444 | rlcv-miR-rL1-14-5p | -52.6 | NC_006146.1 | - | 48409 | 0.77 | 0.441299 |
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Target: 5'- -cCAGGCGCGCCAGGCCcUCCa- -3' miRNA: 3'- uaGUUCGCGUGGUCUGGcAGGcu -5' |
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| MIMAT0003444 | rlcv-miR-rL1-14-5p | -52.6 | NC_006146.1 | - | 142891 | 1.01 | 0.013856 |
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Target: 5'- cAUCAAGCGCACCAGACCGUCCGAc -3' miRNA: 3'- -UAGUUCGCGUGGUCUGGCAGGCU- -5' |
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| MIMAT0003444 | rlcv-miR-rL1-14-5p | -52.6 | NC_006146.1 | - | 143083 | 1.01 | 0.013856 |
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Target: 5'- cAUCAAGCGCACCAGACCGUCCGAc -3' miRNA: 3'- -UAGUUCGCGUGGUCUGGCAGGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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