miRNA: 3'- -GGAac---AUAcGGACGCAGGAGAa -5'
miRNA: 3'- -GGAaCauaCGGACGCAGGAGAa -5'
miRNA: 3'- gGAAcAUACGGACGcAGGAGaa -5'
miRNA: 3'- -GGAACAUACGGAC-GCAGGaGAA- -5'
miRNA: 3'- -GGAACAUACGGACGCAGGAGAA- -5'
Results 1 - 5 of 5 are showing below:
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| Acc. No. | Name | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| MIMAT0003715 | ebv-miR-BART17-5p | -47.2 | NC_007605.1 | - | 155361 | 0.67 | 0.996912 |
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Target: 5'- uCCUgcagaUAU-CCUGCGUCCUCUg -3' miRNA: 3'- -GGAac---AUAcGGACGCAGGAGAa -5' |
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| MIMAT0003715 | ebv-miR-BART17-5p | -47.2 | NC_007605.1 | + | 68787 | 0.68 | 0.993951 |
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Target: 5'- cCCUgGgg-GCCUGUGUCUUCUg -3' miRNA: 3'- -GGAaCauaCGGACGCAGGAGAa -5' |
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| MIMAT0003715 | ebv-miR-BART17-5p | -47.2 | NC_007605.1 | - | 166874 | 0.68 | 0.993951 |
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Target: 5'- aCUUuUAUGCCUGC-UCCUCa- -3' miRNA: 3'- gGAAcAUACGGACGcAGGAGaa -5' |
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| MIMAT0003715 | ebv-miR-BART17-5p | -47.2 | NC_007605.1 | + | 139809 | 0.79 | 0.65204 |
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Target: 5'- uCCUUGUAUGCCUGgUGUCCcCUUa -3' miRNA: 3'- -GGAACAUACGGAC-GCAGGaGAA- -5' |
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| MIMAT0003715 | ebv-miR-BART17-5p | -47.2 | NC_007605.1 | - | 139969 | 1.02 | 0.0346 |
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Target: 5'- aCCUUGUAUGCCUGCGUCCUCUUa -3' miRNA: 3'- -GGAACAUACGGACGCAGGAGAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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