Results 1 - 9 of 9 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
102 | 5' | -60.9 | AC_000006.1 | + | 16344 | 0.66 | 0.287301 |
Target: 5'- -cUGAAuGUCCACggUCUGCacgcccaGGCCCGGCGc -3' miRNA: 3'- acACUU-CAGGUG--GGGCG-------UCGGGCCGU- -5' |
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102 | 5' | -60.9 | AC_000006.1 | + | 15821 | 0.67 | 0.253512 |
Target: 5'- uUGUGcgcGAGUUCGCUCCGCGGC--GGCGc -3' miRNA: 3'- -ACAC---UUCAGGUGGGGCGUCGggCCGU- -5' |
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102 | 5' | -60.9 | AC_000006.1 | + | 25567 | 0.68 | 0.222471 |
Target: 5'- gGUGGaguAGUCCugggagGCgCCCGCGGCCaGGCc -3' miRNA: 3'- aCACU---UCAGG------UG-GGGCGUCGGgCCGu -5' |
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102 | 5' | -60.9 | AC_000006.1 | + | 22734 | 0.68 | 0.210988 |
Target: 5'- --aGAGGccacUCCGCCCCGgGGCCaCGGg- -3' miRNA: 3'- acaCUUC----AGGUGGGGCgUCGG-GCCgu -5' |
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102 | 5' | -60.9 | AC_000006.1 | + | 6520 | 0.69 | 0.179079 |
Target: 5'- cGUGggGUCCGCCCCucaaccccuacgaGCGagcaauggccGCCCgcgaguGGCAg -3' miRNA: 3'- aCACuuCAGGUGGGG-------------CGU----------CGGG------CCGU- -5' |
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102 | 5' | -60.9 | AC_000006.1 | + | 3599 | 0.71 | 0.132613 |
Target: 5'- cGUGggGaacUCgucgcuugacagCACCgCCGCAGCCgCGGCAg -3' miRNA: 3'- aCACuuC---AG------------GUGG-GGCGUCGG-GCCGU- -5' |
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102 | 5' | -60.9 | AC_000006.1 | + | 13391 | 0.71 | 0.131139 |
Target: 5'- gGUGggGUCaacccgauaucgcgCAUCCUGCAGCCCaaacuggGGCGa -3' miRNA: 3'- aCACuuCAG--------------GUGGGGCGUCGGG-------CCGU- -5' |
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102 | 5' | -60.9 | AC_000006.1 | + | 24874 | 0.76 | 0.057842 |
Target: 5'- ------aUCCGCgCCGCAGCCCGGCGg -3' miRNA: 3'- acacuucAGGUGgGGCGUCGGGCCGU- -5' |
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102 | 5' | -60.9 | AC_000006.1 | + | 14269 | 1.08 | 0.000166 |
Target: 5'- cUGUGAAGUCCACCCCGCAGCCCGGCAg -3' miRNA: 3'- -ACACUUCAGGUGGGGCGUCGGGCCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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