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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 10206 | 3' | -50.9 | NC_002665.1 | + | 13794 | 0.66 | 0.974872 |
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Target: 5'- gAGGAUGAucgucACGCCCCcaaUGggGUUu -3' miRNA: 3'- -UCCUACUcu---UGUGGGGaagACuuCAG- -5' |
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| 10206 | 3' | -50.9 | NC_002665.1 | + | 56184 | 0.67 | 0.958226 |
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Target: 5'- ----gGAGAACACCCCUg--GAuGUCu -3' miRNA: 3'- uccuaCUCUUGUGGGGAagaCUuCAG- -5' |
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| 10206 | 3' | -50.9 | NC_002665.1 | + | 90179 | 0.67 | 0.958226 |
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Target: 5'- -aGAUGAcAACACCCCUUCaguuaagaUGAAuGUCc -3' miRNA: 3'- ucCUACUcUUGUGGGGAAG--------ACUU-CAG- -5' |
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| 10206 | 3' | -50.9 | NC_002665.1 | + | 6017 | 0.68 | 0.935256 |
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Target: 5'- --aGUGAGAGCACUCCUcugacCUGAAGcUCu -3' miRNA: 3'- uccUACUCUUGUGGGGAa----GACUUC-AG- -5' |
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| 10206 | 3' | -50.9 | NC_002665.1 | + | 14619 | 0.71 | 0.808383 |
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Target: 5'- gGGGAUGucuGGGCACCUa-UCUGggGUg -3' miRNA: 3'- -UCCUACu--CUUGUGGGgaAGACuuCAg -5' |
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| 10206 | 3' | -50.9 | NC_002665.1 | + | 10719 | 0.73 | 0.738715 |
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Target: 5'- ---cUGAGAAUAUCUgUUCUGAGGUCg -3' miRNA: 3'- uccuACUCUUGUGGGgAAGACUUCAG- -5' |
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| 10206 | 3' | -50.9 | NC_002665.1 | + | 59464 | 1.1 | 0.005187 |
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Target: 5'- gAGGAUGAGAACACCCCUUCUGAAGUCc -3' miRNA: 3'- -UCCUACUCUUGUGGGGAAGACUUCAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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