Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 10207 | 3' | -56.3 | NC_002665.1 | + | 68524 | 1.08 | 0.001788 |
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Target: 5'- gACCCAGGAGUGGGCAGGAGACAUUGAu -3' miRNA: 3'- -UGGGUCCUCACCCGUCCUCUGUAACU- -5' |
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| 10207 | 3' | -56.3 | NC_002665.1 | + | 38045 | 0.69 | 0.664705 |
|
Target: 5'- -gCCAGGAGUGGGU-GGAG-UGUUGc -3' miRNA: 3'- ugGGUCCUCACCCGuCCUCuGUAACu -5' |
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| 10207 | 3' | -56.3 | NC_002665.1 | + | 108559 | 0.68 | 0.692901 |
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Target: 5'- uGCCCAGGGcGUacugguacugugugGGGUGGGGGACGagGGc -3' miRNA: 3'- -UGGGUCCU-CA--------------CCCGUCCUCUGUaaCU- -5' |
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| 10207 | 3' | -56.3 | NC_002665.1 | + | 68351 | 0.67 | 0.746927 |
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Target: 5'- gGCCCAu---UGGGCguugGGGGGGCAUUGAc -3' miRNA: 3'- -UGGGUccucACCCG----UCCUCUGUAACU- -5' |
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| 10207 | 3' | -56.3 | NC_002665.1 | + | 96347 | 0.66 | 0.795175 |
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Target: 5'- aACCCGGGGaagGGGaAGGAGGCuaggGAa -3' miRNA: 3'- -UGGGUCCUca-CCCgUCCUCUGuaa-CU- -5' |
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| 10207 | 3' | -56.3 | NC_002665.1 | + | 96375 | 0.66 | 0.795175 |
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Target: 5'- aACCCGGGGaagGGGaAGGAGGCuaggGAa -3' miRNA: 3'- -UGGGUCCUca-CCCgUCCUCUGuaa-CU- -5' |
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| 10207 | 3' | -56.3 | NC_002665.1 | + | 96782 | 0.66 | 0.795175 |
|
Target: 5'- cCCCGGGGaaGGGgaaGGGAGGCGggGAa -3' miRNA: 3'- uGGGUCCUcaCCCg--UCCUCUGUaaCU- -5' |
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| 10207 | 3' | -56.3 | NC_002665.1 | + | 99970 | 0.66 | 0.83103 |
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Target: 5'- cCCCacgucuaugAGGGcGUGGGCcccgacuuGGGGGCGUUGGu -3' miRNA: 3'- uGGG---------UCCU-CACCCGu-------CCUCUGUAACU- -5' |
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| 10207 | 3' | -56.3 | NC_002665.1 | + | 98678 | 0.66 | 0.804396 |
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Target: 5'- uACCCcguGGAGUGGGUuguGGGGGugugugGCAcgUGAc -3' miRNA: 3'- -UGGGu--CCUCACCCG---UCCUC------UGUa-ACU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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