Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 10219 | 3' | -49.7 | NC_002665.1 | + | 2668 | 1.07 | 0.00885 |
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Target: 5'- cAAUAAAGGGGGCACAUGUGGCUAUAGu -3' miRNA: 3'- -UUAUUUCCCCCGUGUACACCGAUAUC- -5' |
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| 10219 | 3' | -49.7 | NC_002665.1 | + | 5988 | 0.67 | 0.977599 |
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Target: 5'- ----uAGGcaccaauaGGGC-CAUGUGGCUAUAu -3' miRNA: 3'- uuauuUCC--------CCCGuGUACACCGAUAUc -5' |
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| 10219 | 3' | -49.7 | NC_002665.1 | + | 23770 | 0.67 | 0.974896 |
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Target: 5'- gGGUGAguuGGGGGGCGCucugGUGGggGuUGGg -3' miRNA: 3'- -UUAUU---UCCCCCGUGua--CACCgaU-AUC- -5' |
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| 10219 | 3' | -49.7 | NC_002665.1 | + | 24860 | 0.67 | 0.968793 |
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Target: 5'- --cGGAGGGGGCG--UGUGGuCUGg-- -3' miRNA: 3'- uuaUUUCCCCCGUguACACC-GAUauc -5' |
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| 10219 | 3' | -49.7 | NC_002665.1 | + | 108581 | 0.67 | 0.968793 |
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Target: 5'- uGUGGGGuGGGGgACGaG-GGCUGUGGa -3' miRNA: 3'- uUAUUUC-CCCCgUGUaCaCCGAUAUC- -5' |
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| 10219 | 3' | -49.7 | NC_002665.1 | + | 73503 | 0.68 | 0.961701 |
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Target: 5'- --gGAGGGGGGUgACAUcccGUGGCaccAUAGa -3' miRNA: 3'- uuaUUUCCCCCG-UGUA---CACCGa--UAUC- -5' |
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| 10219 | 3' | -49.7 | NC_002665.1 | + | 70499 | 0.82 | 0.297827 |
|
Target: 5'- --aAGAGGGGGCGCGUGUGGgUGa-- -3' miRNA: 3'- uuaUUUCCCCCGUGUACACCgAUauc -5' |
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| 10219 | 3' | -49.7 | NC_002665.1 | + | 89956 | 0.68 | 0.957766 |
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Target: 5'- -uUAGAGGGGaGacUugGUGUGGCUGa-- -3' miRNA: 3'- uuAUUUCCCC-C--GugUACACCGAUauc -5' |
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| 10219 | 3' | -49.7 | NC_002665.1 | + | 6573 | 0.67 | 0.965374 |
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Target: 5'- --cAAGGaGGGGaauCAUGUGGCcAUGGu -3' miRNA: 3'- uuaUUUC-CCCCgu-GUACACCGaUAUC- -5' |
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| 10219 | 3' | -49.7 | NC_002665.1 | + | 108329 | 0.66 | 0.984421 |
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Target: 5'- -cUGAAGGGuGGCcCAgagGUguucgucggGGCUAUAGg -3' miRNA: 3'- uuAUUUCCC-CCGuGUa--CA---------CCGAUAUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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