Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 10221 | 5' | -50.2 | NC_002665.1 | + | 8655 | 0.66 | 0.985948 |
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Target: 5'- cGGGACUGUCCUcacagUAGGCaccagGCaaUGGaGAGu -3' miRNA: 3'- -CCCUGACAGGA-----AUUUGa----CGa-ACC-CUU- -5' |
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| 10221 | 5' | -50.2 | NC_002665.1 | + | 88485 | 0.68 | 0.948513 |
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Target: 5'- gGGGuuUGUUCUUGGACuuguucaUGuCUUGGGAGg -3' miRNA: 3'- -CCCugACAGGAAUUUG-------AC-GAACCCUU- -5' |
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| 10221 | 5' | -50.2 | NC_002665.1 | + | 89252 | 0.69 | 0.934073 |
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Target: 5'- gGGGGCUuggaGUCCUcgagaUGGuCUGUUUGGGGu -3' miRNA: 3'- -CCCUGA----CAGGA-----AUUuGACGAACCCUu -5' |
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| 10221 | 5' | -50.2 | NC_002665.1 | + | 108512 | 0.72 | 0.804594 |
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Target: 5'- uGGGACUGaaCUUUGAGCUGCacugUGGGc- -3' miRNA: 3'- -CCCUGACa-GGAAUUUGACGa---ACCCuu -5' |
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| 10221 | 5' | -50.2 | NC_002665.1 | + | 89208 | 0.75 | 0.635837 |
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Target: 5'- uGGGugUGUCUgu-AACUGCggGGGGGu -3' miRNA: 3'- -CCCugACAGGaauUUGACGaaCCCUU- -5' |
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| 10221 | 5' | -50.2 | NC_002665.1 | + | 14541 | 1.09 | 0.006132 |
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Target: 5'- uGGGACUGUCCUUAAACUGCUUGGGAAg -3' miRNA: 3'- -CCCUGACAGGAAUUUGACGAACCCUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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