miRNA: 3'- -GGUACUA--ACC---GACUGaCGGUa-CUCCU- -5'
miRNA: 3'- -GGUACUAACCGACUGACGGUACUCCU- -5'
miRNA: 3'- -GGuACUAACCGAcUGACGGuACUccu -5'
miRNA: 3'- -GGUACUAACCGACugaCGGuACuCCU- -5'
miRNA: 3'- gGUAc---UAACCGAC--UGACGGUACUccu -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 10223 | 3' | -51.7 | NC_002665.1 | + | 21169 | 0.7 | 0.835971 |
|
Target: 5'- uCCAUGGUgaUGGaauCUGACaGCCAagGGGGAg -3' miRNA: 3'- -GGUACUA--ACC---GACUGaCGGUa-CUCCU- -5' |
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| 10223 | 3' | -51.7 | NC_002665.1 | + | 21232 | 1.11 | 0.003623 |
|
Target: 5'- uCCAUGAUUGGCUGACUGCCAUGAGGAc -3' miRNA: 3'- -GGUACUAACCGACUGACGGUACUCCU- -5' |
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| 10223 | 3' | -51.7 | NC_002665.1 | + | 21403 | 0.67 | 0.944706 |
|
Target: 5'- aCC-UGAUUGGCUaACUGCCuUGAc-- -3' miRNA: 3'- -GGuACUAACCGAcUGACGGuACUccu -5' |
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| 10223 | 3' | -51.7 | NC_002665.1 | + | 66916 | 0.73 | 0.708569 |
|
Target: 5'- uCCAUGGUUGGCUGg--GCCuUG-GGAu -3' miRNA: 3'- -GGUACUAACCGACugaCGGuACuCCU- -5' |
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| 10223 | 3' | -51.7 | NC_002665.1 | + | 101331 | 0.67 | 0.944706 |
|
Target: 5'- aCAUugucAUUGGCUGuuGCUGCCAUGAu-- -3' miRNA: 3'- gGUAc---UAACCGAC--UGACGGUACUccu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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