Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
10248 | 5' | -65.5 | NC_002686.1 | + | 120648 | 0.7 | 0.266335 |
Target: 5'- uGuuCCACGGGUCCCG-GGUUCCUa- -3' miRNA: 3'- gCugGGUGCCCGGGGCuCCAGGGGcc -5' |
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10248 | 5' | -65.5 | NC_002686.1 | + | 107507 | 0.7 | 0.266335 |
Target: 5'- uGuuCCACGGGUCCCG-GGUUCCUa- -3' miRNA: 3'- gCugGGUGCCCGGGGCuCCAGGGGcc -5' |
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10248 | 5' | -65.5 | NC_002686.1 | + | 104163 | 0.72 | 0.222465 |
Target: 5'- gGACCCGggagaGGGCCggggggggCCGGGGaccCCCCGGg -3' miRNA: 3'- gCUGGGUg----CCCGG--------GGCUCCa--GGGGCC- -5' |
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10248 | 5' | -65.5 | NC_002686.1 | + | 120153 | 1.08 | 0.000508 |
Target: 5'- gCGACCCACGGGCCCCGAGGUCCCCGGc -3' miRNA: 3'- -GCUGGGUGCCCGGGGCUCCAGGGGCC- -5' |
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10248 | 5' | -65.5 | NC_002686.1 | + | 108002 | 1.08 | 0.000508 |
Target: 5'- gCGACCCACGGGCCCCGAGGUCCCCGGc -3' miRNA: 3'- -GCUGGGUGCCCGGGGCUCCAGGGGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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