Results 1 - 20 of 28 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
10292 | 3' | -51.7 | NC_002687.1 | + | 315632 | 0.66 | 0.999188 |
Target: 5'- aGCCGCAugaaGCUUGGCugUcaccACcgUCc -3' miRNA: 3'- -CGGCGUug--CGAACCGugAc---UGuaAGu -5' |
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10292 | 3' | -51.7 | NC_002687.1 | + | 103401 | 0.66 | 0.999015 |
Target: 5'- gGCUGCAGC-CUUGGCGgccUUGGCug-CAg -3' miRNA: 3'- -CGGCGUUGcGAACCGU---GACUGuaaGU- -5' |
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10292 | 3' | -51.7 | NC_002687.1 | + | 145831 | 0.66 | 0.998812 |
Target: 5'- cGCCGaGGCGCgcgccggGGCGCUGGg--UCAg -3' miRNA: 3'- -CGGCgUUGCGaa-----CCGUGACUguaAGU- -5' |
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10292 | 3' | -51.7 | NC_002687.1 | + | 155149 | 0.66 | 0.998812 |
Target: 5'- cGCCGaCGACGCggugucuacGGCGgUGugcACAUUCGg -3' miRNA: 3'- -CGGC-GUUGCGaa-------CCGUgAC---UGUAAGU- -5' |
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10292 | 3' | -51.7 | NC_002687.1 | + | 244817 | 0.66 | 0.998812 |
Target: 5'- uGCCGCAACGgg-GGCACcGugAa--- -3' miRNA: 3'- -CGGCGUUGCgaaCCGUGaCugUaagu -5' |
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10292 | 3' | -51.7 | NC_002687.1 | + | 59488 | 0.67 | 0.997977 |
Target: 5'- aCCGCGACuCUUGGCGa-GACAUg-- -3' miRNA: 3'- cGGCGUUGcGAACCGUgaCUGUAagu -5' |
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10292 | 3' | -51.7 | NC_002687.1 | + | 201849 | 0.67 | 0.997977 |
Target: 5'- uCCaGCGGCgGCUUGGCACaGugGcUCAc -3' miRNA: 3'- cGG-CGUUG-CGAACCGUGaCugUaAGU- -5' |
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10292 | 3' | -51.7 | NC_002687.1 | + | 238125 | 0.67 | 0.997977 |
Target: 5'- aGgCGCAcauCGCcaUGGuCGCUGGCAUUCc -3' miRNA: 3'- -CgGCGUu--GCGa-ACC-GUGACUGUAAGu -5' |
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10292 | 3' | -51.7 | NC_002687.1 | + | 298329 | 0.68 | 0.995519 |
Target: 5'- gGCCGCAACGCaagGGCGuuuguUUGAUGUg-- -3' miRNA: 3'- -CGGCGUUGCGaa-CCGU-----GACUGUAagu -5' |
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10292 | 3' | -51.7 | NC_002687.1 | + | 25569 | 0.68 | 0.995519 |
Target: 5'- uGCCGCAgaaccaGCgGCUUGGCACaaccgaGACAcgCGa -3' miRNA: 3'- -CGGCGU------UG-CGAACCGUGa-----CUGUaaGU- -5' |
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10292 | 3' | -51.7 | NC_002687.1 | + | 87708 | 0.68 | 0.992139 |
Target: 5'- cGCCG-GGCGUgUGGCGCUGACc---- -3' miRNA: 3'- -CGGCgUUGCGaACCGUGACUGuaagu -5' |
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10292 | 3' | -51.7 | NC_002687.1 | + | 134064 | 0.68 | 0.992139 |
Target: 5'- aCCGC-GCGCUcuUGGCACcgUGGCG-UCAc -3' miRNA: 3'- cGGCGuUGCGA--ACCGUG--ACUGUaAGU- -5' |
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10292 | 3' | -51.7 | NC_002687.1 | + | 224149 | 0.68 | 0.992139 |
Target: 5'- uGgCGCuGGCGC-UGGCGCUGGCGguggCGg -3' miRNA: 3'- -CgGCG-UUGCGaACCGUGACUGUaa--GU- -5' |
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10292 | 3' | -51.7 | NC_002687.1 | + | 135619 | 0.69 | 0.989817 |
Target: 5'- aCCGCGACGCggugcagcgguUUGGCAacggggacauCUGGCAggCAu -3' miRNA: 3'- cGGCGUUGCG-----------AACCGU----------GACUGUaaGU- -5' |
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10292 | 3' | -51.7 | NC_002687.1 | + | 205047 | 0.69 | 0.989817 |
Target: 5'- aGUCGCGACGUUgcGGCACauggUGACGUcCGu -3' miRNA: 3'- -CGGCGUUGCGAa-CCGUG----ACUGUAaGU- -5' |
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10292 | 3' | -51.7 | NC_002687.1 | + | 44252 | 0.69 | 0.98699 |
Target: 5'- cUCGCAGCGUUuuuuaUGGaCGCUGGC-UUCAu -3' miRNA: 3'- cGGCGUUGCGA-----ACC-GUGACUGuAAGU- -5' |
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10292 | 3' | -51.7 | NC_002687.1 | + | 238151 | 0.69 | 0.985197 |
Target: 5'- aCUGCGAaaacaagUGCUUGGCGCacaugacgacgaUGACGUUCGu -3' miRNA: 3'- cGGCGUU-------GCGAACCGUG------------ACUGUAAGU- -5' |
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10292 | 3' | -51.7 | NC_002687.1 | + | 298372 | 0.7 | 0.977299 |
Target: 5'- uGCCGCGACGCUcgagcUGuGCuuUGAUAUUUc -3' miRNA: 3'- -CGGCGUUGCGA-----AC-CGugACUGUAAGu -5' |
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10292 | 3' | -51.7 | NC_002687.1 | + | 255866 | 0.7 | 0.972213 |
Target: 5'- aGCC-CAACaGUgaggUGGCACUGACAgaCAu -3' miRNA: 3'- -CGGcGUUG-CGa---ACCGUGACUGUaaGU- -5' |
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10292 | 3' | -51.7 | NC_002687.1 | + | 224853 | 0.71 | 0.963117 |
Target: 5'- aGCCGcCGACGCggcGGCGCcGGCAcggUCGg -3' miRNA: 3'- -CGGC-GUUGCGaa-CCGUGaCUGUa--AGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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