Results 1 - 20 of 35 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
10293 | 3' | -50.2 | NC_002687.1 | + | 175744 | 0.66 | 0.999869 |
Target: 5'- gGGAuuGCGACGUCAAGCuucGUAGGggUGUa -3' miRNA: 3'- gCCU--CGUUGCGGUUCGu--CAUUUa-GCA- -5' |
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10293 | 3' | -50.2 | NC_002687.1 | + | 22169 | 0.66 | 0.999869 |
Target: 5'- gGGAGCAu--UCAGGCAGUGuccGUCGc -3' miRNA: 3'- gCCUCGUugcGGUUCGUCAUu--UAGCa -5' |
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10293 | 3' | -50.2 | NC_002687.1 | + | 18835 | 0.66 | 0.999833 |
Target: 5'- aGGAGCAcuaGCaCGAGCGGcuucauGUCGUc -3' miRNA: 3'- gCCUCGUug-CG-GUUCGUCauu---UAGCA- -5' |
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10293 | 3' | -50.2 | NC_002687.1 | + | 71767 | 0.66 | 0.999833 |
Target: 5'- aCGGAGUacuuGACGCCGuauGCGGUGGc---- -3' miRNA: 3'- -GCCUCG----UUGCGGUu--CGUCAUUuagca -5' |
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10293 | 3' | -50.2 | NC_002687.1 | + | 2643 | 0.66 | 0.999833 |
Target: 5'- gGGAGguGCGUgGAGUggugucAGUAGGUUGa -3' miRNA: 3'- gCCUCguUGCGgUUCG------UCAUUUAGCa -5' |
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10293 | 3' | -50.2 | NC_002687.1 | + | 303870 | 0.66 | 0.999833 |
Target: 5'- cCGGAGCAACcguaGCUGcagcAGCAGUAGcgCu- -3' miRNA: 3'- -GCCUCGUUG----CGGU----UCGUCAUUuaGca -5' |
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10293 | 3' | -50.2 | NC_002687.1 | + | 48070 | 0.66 | 0.999789 |
Target: 5'- uCGGAGCAgcauguACGUgcAGCAGUu-AUCGg -3' miRNA: 3'- -GCCUCGU------UGCGguUCGUCAuuUAGCa -5' |
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10293 | 3' | -50.2 | NC_002687.1 | + | 60621 | 0.66 | 0.999789 |
Target: 5'- aCGGcaaAGCGA-GCCAAGCAGaacgAAGUgGUg -3' miRNA: 3'- -GCC---UCGUUgCGGUUCGUCa---UUUAgCA- -5' |
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10293 | 3' | -50.2 | NC_002687.1 | + | 119532 | 0.66 | 0.999768 |
Target: 5'- aGGAGC-GCGCCAGGUuugaaaaccuGGUCGg -3' miRNA: 3'- gCCUCGuUGCGGUUCGucau------UUAGCa -5' |
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10293 | 3' | -50.2 | NC_002687.1 | + | 73072 | 0.66 | 0.999734 |
Target: 5'- aCGGA-CGAugcUGCCGAGCGG-GGGUCGUc -3' miRNA: 3'- -GCCUcGUU---GCGGUUCGUCaUUUAGCA- -5' |
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10293 | 3' | -50.2 | NC_002687.1 | + | 241610 | 0.67 | 0.999585 |
Target: 5'- uCGGAGCAG-GCCAAGaAGUAcGAUCc- -3' miRNA: 3'- -GCCUCGUUgCGGUUCgUCAU-UUAGca -5' |
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10293 | 3' | -50.2 | NC_002687.1 | + | 204193 | 0.67 | 0.999585 |
Target: 5'- gGGGGCggUGCCGaacAGCAcgGUGGAuuUCGa -3' miRNA: 3'- gCCUCGuuGCGGU---UCGU--CAUUU--AGCa -5' |
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10293 | 3' | -50.2 | NC_002687.1 | + | 312223 | 0.67 | 0.999487 |
Target: 5'- aCGGaAGCgAGCgGCgGAGCAGcgGGAUCGg -3' miRNA: 3'- -GCC-UCG-UUG-CGgUUCGUCa-UUUAGCa -5' |
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10293 | 3' | -50.2 | NC_002687.1 | + | 35818 | 0.67 | 0.999487 |
Target: 5'- aGGGGCGGgcaucUGCCGAuGCGGUGAuacguUCGa -3' miRNA: 3'- gCCUCGUU-----GCGGUU-CGUCAUUu----AGCa -5' |
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10293 | 3' | -50.2 | NC_002687.1 | + | 305249 | 0.67 | 0.999487 |
Target: 5'- aGGAGU--UGCCGAGCAGauGAUCc- -3' miRNA: 3'- gCCUCGuuGCGGUUCGUCauUUAGca -5' |
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10293 | 3' | -50.2 | NC_002687.1 | + | 316377 | 0.67 | 0.999369 |
Target: 5'- aCGGAGCAacACGCgAAGC-GUGAAg--- -3' miRNA: 3'- -GCCUCGU--UGCGgUUCGuCAUUUagca -5' |
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10293 | 3' | -50.2 | NC_002687.1 | + | 161474 | 0.67 | 0.999369 |
Target: 5'- uGGAuCAugGCCAcGCGGccgGGAUCGa -3' miRNA: 3'- gCCUcGUugCGGUuCGUCa--UUUAGCa -5' |
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10293 | 3' | -50.2 | NC_002687.1 | + | 255041 | 0.67 | 0.999228 |
Target: 5'- uGG-GCAACGCCGAGgcccucCAGUAcAUUGa -3' miRNA: 3'- gCCuCGUUGCGGUUC------GUCAUuUAGCa -5' |
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10293 | 3' | -50.2 | NC_002687.1 | + | 210681 | 0.67 | 0.999061 |
Target: 5'- gCGG-GCgAGCGCCAGaacGCAG-AGGUCGg -3' miRNA: 3'- -GCCuCG-UUGCGGUU---CGUCaUUUAGCa -5' |
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10293 | 3' | -50.2 | NC_002687.1 | + | 47640 | 0.68 | 0.998865 |
Target: 5'- gGGAGCGuuUGCUcAGCAGUAGGUUu- -3' miRNA: 3'- gCCUCGUu-GCGGuUCGUCAUUUAGca -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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