Results 1 - 20 of 27 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
10299 | 5' | -55 | NC_002687.1 | + | 209286 | 0.66 | 0.990445 |
Target: 5'- gACCAcCCgggugguuuuucuuaCACuGUGUCgCCGCGGGAUAc -3' miRNA: 3'- -UGGUuGG---------------GUGuCAUAG-GGCGCUCUGU- -5' |
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10299 | 5' | -55 | NC_002687.1 | + | 85664 | 0.66 | 0.988669 |
Target: 5'- gACCGacgggACCCACGGga-CCCaCGGGACc -3' miRNA: 3'- -UGGU-----UGGGUGUCauaGGGcGCUCUGu -5' |
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10299 | 5' | -55 | NC_002687.1 | + | 200149 | 0.66 | 0.98725 |
Target: 5'- uGCCAAUcgccaCCACcGUAUCCCcuCGGGACc -3' miRNA: 3'- -UGGUUG-----GGUGuCAUAGGGc-GCUCUGu -5' |
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10299 | 5' | -55 | NC_002687.1 | + | 84277 | 0.66 | 0.984007 |
Target: 5'- gACUGACCCACAGaucgacuuUGUCCuCG-GGGACc -3' miRNA: 3'- -UGGUUGGGUGUC--------AUAGG-GCgCUCUGu -5' |
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10299 | 5' | -55 | NC_002687.1 | + | 39038 | 0.66 | 0.983471 |
Target: 5'- -gCAGCCCGCGaaggccacgcucauGUcgCCCGCGAGcuGCu -3' miRNA: 3'- ugGUUGGGUGU--------------CAuaGGGCGCUC--UGu -5' |
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10299 | 5' | -55 | NC_002687.1 | + | 111245 | 0.67 | 0.982168 |
Target: 5'- -aCGACgUCugGGUAUCCUGCGAGuuGg -3' miRNA: 3'- ugGUUG-GGugUCAUAGGGCGCUCugU- -5' |
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10299 | 5' | -55 | NC_002687.1 | + | 85277 | 0.67 | 0.982168 |
Target: 5'- cGCUGGCCCACaAGgccucccugGUCCCGUGGGuCc -3' miRNA: 3'- -UGGUUGGGUG-UCa--------UAGGGCGCUCuGu -5' |
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10299 | 5' | -55 | NC_002687.1 | + | 98006 | 0.67 | 0.978018 |
Target: 5'- aACC-ACCCGuCGGUGUCaCCGCc-GACAc -3' miRNA: 3'- -UGGuUGGGU-GUCAUAG-GGCGcuCUGU- -5' |
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10299 | 5' | -55 | NC_002687.1 | + | 297593 | 0.67 | 0.973196 |
Target: 5'- gGCCAACCgugACA---UCCCGaCGAGGCAc -3' miRNA: 3'- -UGGUUGGg--UGUcauAGGGC-GCUCUGU- -5' |
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10299 | 5' | -55 | NC_002687.1 | + | 184290 | 0.67 | 0.973196 |
Target: 5'- cGCCAGCUggaaACAGguuguuauUCuuGCGAGGCAg -3' miRNA: 3'- -UGGUUGGg---UGUCau------AGggCGCUCUGU- -5' |
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10299 | 5' | -55 | NC_002687.1 | + | 89698 | 0.68 | 0.967651 |
Target: 5'- uGCCGuguCCACGGUGUCuCCGcCGAGGuCGg -3' miRNA: 3'- -UGGUug-GGUGUCAUAG-GGC-GCUCU-GU- -5' |
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10299 | 5' | -55 | NC_002687.1 | + | 293927 | 0.68 | 0.964593 |
Target: 5'- -aCAAgCCAUucguGUAUUCCGCGAGAgGg -3' miRNA: 3'- ugGUUgGGUGu---CAUAGGGCGCUCUgU- -5' |
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10299 | 5' | -55 | NC_002687.1 | + | 209678 | 0.68 | 0.961338 |
Target: 5'- uCCGGaucaaCAgGGUAUCCCGCGGcGACAc -3' miRNA: 3'- uGGUUgg---GUgUCAUAGGGCGCU-CUGU- -5' |
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10299 | 5' | -55 | NC_002687.1 | + | 285203 | 0.69 | 0.950348 |
Target: 5'- aGCCAAUCCACccgGUCCC-CGGGAUc -3' miRNA: 3'- -UGGUUGGGUGucaUAGGGcGCUCUGu -5' |
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10299 | 5' | -55 | NC_002687.1 | + | 308328 | 0.69 | 0.937449 |
Target: 5'- uUCGACCCGuguGUcUCUCGCGGGGCAa -3' miRNA: 3'- uGGUUGGGUgu-CAuAGGGCGCUCUGU- -5' |
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10299 | 5' | -55 | NC_002687.1 | + | 154145 | 0.69 | 0.932715 |
Target: 5'- aGCCAGCCgAguCAGUGUUCCGCGcGuCAc -3' miRNA: 3'- -UGGUUGGgU--GUCAUAGGGCGCuCuGU- -5' |
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10299 | 5' | -55 | NC_002687.1 | + | 201224 | 0.7 | 0.922587 |
Target: 5'- -gCGGCgCCACGcucUAUCCUGCGGGGCAg -3' miRNA: 3'- ugGUUG-GGUGUc--AUAGGGCGCUCUGU- -5' |
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10299 | 5' | -55 | NC_002687.1 | + | 236809 | 0.7 | 0.905751 |
Target: 5'- gUCAACCCugGGaaagucaaGUCCCGUGAGAgAg -3' miRNA: 3'- uGGUUGGGugUCa-------UAGGGCGCUCUgU- -5' |
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10299 | 5' | -55 | NC_002687.1 | + | 317023 | 0.7 | 0.899706 |
Target: 5'- gACC-AUCCGUGGUGUCUCGCGAGAg- -3' miRNA: 3'- -UGGuUGGGUGUCAUAGGGCGCUCUgu -5' |
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10299 | 5' | -55 | NC_002687.1 | + | 210026 | 0.71 | 0.882997 |
Target: 5'- cACCAAUCUGCAuaucaucuucucguuGUGUCUCGCGGGAUc -3' miRNA: 3'- -UGGUUGGGUGU---------------CAUAGGGCGCUCUGu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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