Results 21 - 27 of 27 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
10299 | 5' | -55 | NC_002687.1 | + | 96847 | 0.71 | 0.873423 |
Target: 5'- aACgCGGCCCGC-GUcgCUCGCGGGGCu -3' miRNA: 3'- -UG-GUUGGGUGuCAuaGGGCGCUCUGu -5' |
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10299 | 5' | -55 | NC_002687.1 | + | 227832 | 0.72 | 0.851614 |
Target: 5'- gGCCAccuCCCACGGacAUCCuUGUGAGACAu -3' miRNA: 3'- -UGGUu--GGGUGUCa-UAGG-GCGCUCUGU- -5' |
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10299 | 5' | -55 | NC_002687.1 | + | 85705 | 0.72 | 0.828161 |
Target: 5'- cGCCgGACCgACGGga-CCCGCGGGACc -3' miRNA: 3'- -UGG-UUGGgUGUCauaGGGCGCUCUGu -5' |
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10299 | 5' | -55 | NC_002687.1 | + | 209635 | 0.72 | 0.828161 |
Target: 5'- -gCGACCgGguGcaccgAUCCCGCGAGACAc -3' miRNA: 3'- ugGUUGGgUguCa----UAGGGCGCUCUGU- -5' |
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10299 | 5' | -55 | NC_002687.1 | + | 318603 | 1.08 | 0.008678 |
Target: 5'- aACCAACCCACAGUAUCCCGCGAGACAa -3' miRNA: 3'- -UGGUUGGGUGUCAUAGGGCGCUCUGU- -5' |
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10299 | 5' | -55 | NC_002687.1 | + | 247312 | 1.08 | 0.008678 |
Target: 5'- aACCAACCCACAGUAUCCCGCGAGACAa -3' miRNA: 3'- -UGGUUGGGUGUCAUAGGGCGCUCUGU- -5' |
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10299 | 5' | -55 | NC_002687.1 | + | 40677 | 1.08 | 0.008678 |
Target: 5'- aACCAACCCACAGUAUCCCGCGAGACAa -3' miRNA: 3'- -UGGUUGGGUGUCAUAGGGCGCUCUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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