Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 10299 | 5' | -55 | NC_002687.1 | + | 247312 | 1.08 | 0.008678 |
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Target: 5'- aACCAACCCACAGUAUCCCGCGAGACAa -3' miRNA: 3'- -UGGUUGGGUGUCAUAGGGCGCUCUGU- -5' |
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| 10299 | 5' | -55 | NC_002687.1 | + | 285203 | 0.69 | 0.950348 |
|
Target: 5'- aGCCAAUCCACccgGUCCC-CGGGAUc -3' miRNA: 3'- -UGGUUGGGUGucaUAGGGcGCUCUGu -5' |
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| 10299 | 5' | -55 | NC_002687.1 | + | 293927 | 0.68 | 0.964593 |
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Target: 5'- -aCAAgCCAUucguGUAUUCCGCGAGAgGg -3' miRNA: 3'- ugGUUgGGUGu---CAUAGGGCGCUCUgU- -5' |
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| 10299 | 5' | -55 | NC_002687.1 | + | 297593 | 0.67 | 0.973196 |
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Target: 5'- gGCCAACCgugACA---UCCCGaCGAGGCAc -3' miRNA: 3'- -UGGUUGGg--UGUcauAGGGC-GCUCUGU- -5' |
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| 10299 | 5' | -55 | NC_002687.1 | + | 308328 | 0.69 | 0.937449 |
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Target: 5'- uUCGACCCGuguGUcUCUCGCGGGGCAa -3' miRNA: 3'- uGGUUGGGUgu-CAuAGGGCGCUCUGU- -5' |
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| 10299 | 5' | -55 | NC_002687.1 | + | 317023 | 0.7 | 0.899706 |
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Target: 5'- gACC-AUCCGUGGUGUCUCGCGAGAg- -3' miRNA: 3'- -UGGuUGGGUGUCAUAGGGCGCUCUgu -5' |
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| 10299 | 5' | -55 | NC_002687.1 | + | 318603 | 1.08 | 0.008678 |
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Target: 5'- aACCAACCCACAGUAUCCCGCGAGACAa -3' miRNA: 3'- -UGGUUGGGUGUCAUAGGGCGCUCUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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