Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 10299 | 5' | -55 | NC_002687.1 | + | 85277 | 0.67 | 0.982168 |
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Target: 5'- cGCUGGCCCACaAGgccucccugGUCCCGUGGGuCc -3' miRNA: 3'- -UGGUUGGGUG-UCa--------UAGGGCGCUCuGu -5' |
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| 10299 | 5' | -55 | NC_002687.1 | + | 111245 | 0.67 | 0.982168 |
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Target: 5'- -aCGACgUCugGGUAUCCUGCGAGuuGg -3' miRNA: 3'- ugGUUG-GGugUCAUAGGGCGCUCugU- -5' |
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| 10299 | 5' | -55 | NC_002687.1 | + | 39038 | 0.66 | 0.983471 |
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Target: 5'- -gCAGCCCGCGaaggccacgcucauGUcgCCCGCGAGcuGCu -3' miRNA: 3'- ugGUUGGGUGU--------------CAuaGGGCGCUC--UGu -5' |
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| 10299 | 5' | -55 | NC_002687.1 | + | 84277 | 0.66 | 0.984007 |
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Target: 5'- gACUGACCCACAGaucgacuuUGUCCuCG-GGGACc -3' miRNA: 3'- -UGGUUGGGUGUC--------AUAGG-GCgCUCUGu -5' |
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| 10299 | 5' | -55 | NC_002687.1 | + | 200149 | 0.66 | 0.98725 |
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Target: 5'- uGCCAAUcgccaCCACcGUAUCCCcuCGGGACc -3' miRNA: 3'- -UGGUUG-----GGUGuCAUAGGGc-GCUCUGu -5' |
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| 10299 | 5' | -55 | NC_002687.1 | + | 85664 | 0.66 | 0.988669 |
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Target: 5'- gACCGacgggACCCACGGga-CCCaCGGGACc -3' miRNA: 3'- -UGGU-----UGGGUGUCauaGGGcGCUCUGu -5' |
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| 10299 | 5' | -55 | NC_002687.1 | + | 209286 | 0.66 | 0.990445 |
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Target: 5'- gACCAcCCgggugguuuuucuuaCACuGUGUCgCCGCGGGAUAc -3' miRNA: 3'- -UGGUuGG---------------GUGuCAUAG-GGCGCUCUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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