Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 10311 | 3' | -50.8 | NC_002687.1 | + | 170257 | 0.67 | 0.999038 |
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Target: 5'- -gACCGUUGUugcuGACAUUGUugcugacauUGUUGCUGa -3' miRNA: 3'- aaUGGUAGCG----CUGUAACA---------ACAGCGGC- -5' |
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| 10311 | 3' | -50.8 | NC_002687.1 | + | 97657 | 0.67 | 0.999038 |
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Target: 5'- -cACCGaCGgGugGUUGUUGUUGuuGc -3' miRNA: 3'- aaUGGUaGCgCugUAACAACAGCggC- -5' |
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| 10311 | 3' | -50.8 | NC_002687.1 | + | 296991 | 0.66 | 0.999351 |
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Target: 5'- -cACCAUCGCGcGCGUccucgguggcgaUGUcucgcUGUgCGCCGa -3' miRNA: 3'- aaUGGUAGCGC-UGUA------------ACA-----ACA-GCGGC- -5' |
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| 10311 | 3' | -50.8 | NC_002687.1 | + | 111773 | 0.66 | 0.999472 |
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Target: 5'- cUACuCAUCGUaucGAUAUUcGUUGUCGgCGg -3' miRNA: 3'- aAUG-GUAGCG---CUGUAA-CAACAGCgGC- -5' |
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| 10311 | 3' | -50.8 | NC_002687.1 | + | 182745 | 0.66 | 0.999572 |
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Target: 5'- -cGCCAugugugUCGCGACAUcGa--UCGCCGa -3' miRNA: 3'- aaUGGU------AGCGCUGUAaCaacAGCGGC- -5' |
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| 10311 | 3' | -50.8 | NC_002687.1 | + | 116722 | 0.66 | 0.999572 |
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Target: 5'- -cACCGaCaCGugGUUGUUGUUGUCGu -3' miRNA: 3'- aaUGGUaGcGCugUAACAACAGCGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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