Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 10313 | 5' | -58.7 | NC_002687.1 | + | 45135 | 0.66 | 0.94112 |
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Target: 5'- uGGCGaGCuCGUGGUcaagUCggGUGUGCUUGAg -3' miRNA: 3'- -CCGC-CG-GCGUCA----AGagCGCACGAGCU- -5' |
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| 10313 | 5' | -58.7 | NC_002687.1 | + | 115537 | 0.66 | 0.94112 |
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Target: 5'- cGGUGGCauCGgAGcUCUgGCGUGCguugCGGa -3' miRNA: 3'- -CCGCCG--GCgUCaAGAgCGCACGa---GCU- -5' |
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| 10313 | 5' | -58.7 | NC_002687.1 | + | 292649 | 0.66 | 0.942403 |
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Target: 5'- aGCGGCCGCGGcguucguUUgauguaccacauucgCUCGCG-GCUgGAg -3' miRNA: 3'- cCGCCGGCGUC-------AA---------------GAGCGCaCGAgCU- -5' |
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| 10313 | 5' | -58.7 | NC_002687.1 | + | 200940 | 0.66 | 0.945326 |
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Target: 5'- gGGCGGCUuggcaCAGUggCUCauGCGcGCUCGGg -3' miRNA: 3'- -CCGCCGGc----GUCAa-GAG--CGCaCGAGCU- -5' |
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| 10313 | 5' | -58.7 | NC_002687.1 | + | 175074 | 0.66 | 0.945326 |
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Target: 5'- cGGCGGuuGCAGUagUUccggcgguuccgGCG-GUUCGAg -3' miRNA: 3'- -CCGCCggCGUCAagAG------------CGCaCGAGCU- -5' |
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| 10313 | 5' | -58.7 | NC_002687.1 | + | 35409 | 0.66 | 0.945326 |
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Target: 5'- aGGagGGCCGCAGgggUCGCG-GUuaUCGAg -3' miRNA: 3'- -CCg-CCGGCGUCaagAGCGCaCG--AGCU- -5' |
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| 10313 | 5' | -58.7 | NC_002687.1 | + | 96349 | 0.66 | 0.949329 |
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Target: 5'- uGGauCGGuUCGaGGUUgCUCGCGUGCUUGGu -3' miRNA: 3'- -CC--GCC-GGCgUCAA-GAGCGCACGAGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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