Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 10388 | 3' | -52.5 | NC_002687.1 | + | 226801 | 0.73 | 0.879933 |
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Target: 5'- -cGGUgCCgGUGCCggUGGCGGUGGCGg -3' miRNA: 3'- uaCUA-GG-CACGGuuGCUGUUACCGUa -5' |
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| 10388 | 3' | -52.5 | NC_002687.1 | + | 236598 | 0.69 | 0.966419 |
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Target: 5'- cGUGAUCacaucaagcaGUGCCAACuugcACAGUGGCGc -3' miRNA: 3'- -UACUAGg---------CACGGUUGc---UGUUACCGUa -5' |
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| 10388 | 3' | -52.5 | NC_002687.1 | + | 237752 | 0.7 | 0.963158 |
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Target: 5'- -aGAUCagaGgaaUGCCAGCGACcAUGGCGa -3' miRNA: 3'- uaCUAGg--C---ACGGUUGCUGuUACCGUa -5' |
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| 10388 | 3' | -52.5 | NC_002687.1 | + | 239083 | 1.03 | 0.024271 |
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Target: 5'- cAUGAUCCGUGCCAACGACAAUGGCAUc -3' miRNA: 3'- -UACUAGGCACGGUUGCUGUUACCGUA- -5' |
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| 10388 | 3' | -52.5 | NC_002687.1 | + | 239836 | 0.72 | 0.918141 |
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Target: 5'- cUGGUCCG-GCCGccGCGGCGGaugcUGGCAa -3' miRNA: 3'- uACUAGGCaCGGU--UGCUGUU----ACCGUa -5' |
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| 10388 | 3' | -52.5 | NC_002687.1 | + | 255661 | 0.67 | 0.989968 |
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Target: 5'- --aGUCCGUGUggUGAUGACGGUGGUAg -3' miRNA: 3'- uacUAGGCACG--GUUGCUGUUACCGUa -5' |
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| 10388 | 3' | -52.5 | NC_002687.1 | + | 262956 | 0.68 | 0.983749 |
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Target: 5'- -cGAUCCGcGUCGACaaGAUggUGGCGa -3' miRNA: 3'- uaCUAGGCaCGGUUG--CUGuuACCGUa -5' |
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| 10388 | 3' | -52.5 | NC_002687.1 | + | 296859 | 0.66 | 0.996192 |
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Target: 5'- -cGAUCgCGUGCacuaccuaaaaauCAACGGCGuugGGCAUg -3' miRNA: 3'- uaCUAG-GCACG-------------GUUGCUGUua-CCGUA- -5' |
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| 10388 | 3' | -52.5 | NC_002687.1 | + | 298615 | 0.74 | 0.833919 |
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Target: 5'- -gGAUCCGUGCgCAuCGACGAUGGa-- -3' miRNA: 3'- uaCUAGGCACG-GUuGCUGUUACCgua -5' |
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| 10388 | 3' | -52.5 | NC_002687.1 | + | 316285 | 0.66 | 0.995633 |
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Target: 5'- -gGAUCCG-GUCAugAUGGCGGUGGUg- -3' miRNA: 3'- uaCUAGGCaCGGU--UGCUGUUACCGua -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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