Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 10388 | 5' | -61.8 | NC_002687.1 | + | 258269 | 0.66 | 0.833396 |
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Target: 5'- gUCACGgacaGCGGCGUCGGUcuaagcgaGGCUgACg- -3' miRNA: 3'- -AGUGCg---CGUCGCAGCCA--------CCGGgUGgc -5' |
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| 10388 | 5' | -61.8 | NC_002687.1 | + | 97754 | 0.66 | 0.839395 |
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Target: 5'- -aGCGUugGCGGCGUggauguucucguugcUGGcaccgcuggcaccgcUGGCCCACCGg -3' miRNA: 3'- agUGCG--CGUCGCA---------------GCC---------------ACCGGGUGGC- -5' |
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| 10388 | 5' | -61.8 | NC_002687.1 | + | 236938 | 0.66 | 0.840879 |
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Target: 5'- aUCACGaUGCAcaaGUUGG-GGCCgACCGg -3' miRNA: 3'- -AGUGC-GCGUcg-CAGCCaCCGGgUGGC- -5' |
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| 10388 | 5' | -61.8 | NC_002687.1 | + | 250876 | 0.66 | 0.862363 |
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Target: 5'- gUCcCGCGUGGaGUCGGUGcaaUCCGCCGu -3' miRNA: 3'- -AGuGCGCGUCgCAGCCACc--GGGUGGC- -5' |
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| 10388 | 5' | -61.8 | NC_002687.1 | + | 4438 | 0.66 | 0.862363 |
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Target: 5'- gCACGCGCAGUG-CGGgcacagGGa--GCCGa -3' miRNA: 3'- aGUGCGCGUCGCaGCCa-----CCgggUGGC- -5' |
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| 10388 | 5' | -61.8 | NC_002687.1 | + | 297340 | 0.66 | 0.869187 |
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Target: 5'- aCGCGCGCgauGGUGUCGGcGGacugCUugCGg -3' miRNA: 3'- aGUGCGCG---UCGCAGCCaCCg---GGugGC- -5' |
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| 10388 | 5' | -61.8 | NC_002687.1 | + | 329770 | 0.66 | 0.869187 |
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Target: 5'- aUCGCugcuGCGCcgaGGCGUgccCGGUGGCgUgGCCGg -3' miRNA: 3'- -AGUG----CGCG---UCGCA---GCCACCG-GgUGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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