Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 104 | 5' | -56.8 | AC_000006.1 | + | 6416 | 0.66 | 0.548914 |
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Target: 5'- aUCaUCCAaggccaUCUGCCACuCGcGGGCGGc -3' miRNA: 3'- -AGaAGGUag----AGGUGGUG-GCaCCCGCC- -5' |
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| 104 | 5' | -56.8 | AC_000006.1 | + | 10762 | 0.66 | 0.538058 |
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Target: 5'- cUCUUCCAagUCCAUCucuguCUGUGGcuGUGGc -3' miRNA: 3'- -AGAAGGUagAGGUGGu----GGCACC--CGCC- -5' |
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| 104 | 5' | -56.8 | AC_000006.1 | + | 24807 | 0.67 | 0.474722 |
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Target: 5'- uUCUUCCAUCUCUuCCACUa---GCGGc -3' miRNA: 3'- -AGAAGGUAGAGGuGGUGGcaccCGCC- -5' |
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| 104 | 5' | -56.8 | AC_000006.1 | + | 32355 | 0.67 | 0.434631 |
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Target: 5'- --cUCagcaGUCUCUGCCGCCG-GGGCu- -3' miRNA: 3'- agaAGg---UAGAGGUGGUGGCaCCCGcc -5' |
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| 104 | 5' | -56.8 | AC_000006.1 | + | 4612 | 0.69 | 0.360726 |
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Target: 5'- ---aUCAUgUCCACCugCG-GGGCGa -3' miRNA: 3'- agaaGGUAgAGGUGGugGCaCCCGCc -5' |
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| 104 | 5' | -56.8 | AC_000006.1 | + | 9103 | 0.69 | 0.335361 |
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Target: 5'- cUCUUCUAUUUCUuCCuCUGgGGGCGGu -3' miRNA: 3'- -AGAAGGUAGAGGuGGuGGCaCCCGCC- -5' |
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| 104 | 5' | -56.8 | AC_000006.1 | + | 8722 | 0.7 | 0.311324 |
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Target: 5'- --gUCgCAUCUcgaaugaccucgCCGCCACCGUGGagcgcGCGGg -3' miRNA: 3'- agaAG-GUAGA------------GGUGGUGGCACC-----CGCC- -5' |
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| 104 | 5' | -56.8 | AC_000006.1 | + | 21969 | 0.75 | 0.155938 |
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Target: 5'- --aUCCAUCUCCACCA-CGUGGuccuuGUGGa -3' miRNA: 3'- agaAGGUAGAGGUGGUgGCACC-----CGCC- -5' |
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| 104 | 5' | -56.8 | AC_000006.1 | + | 17382 | 1.1 | 0.00037 |
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Target: 5'- aUCUUCCAUCUCCACCACCGUGGGCGGc -3' miRNA: 3'- -AGAAGGUAGAGGUGGUGGCACCCGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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