Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 10424 | 3' | -57.5 | NC_002687.1 | + | 113738 | 0.66 | 0.942857 |
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Target: 5'- --uUACCGCUGCCGCAgcgACGCuuuGCu -3' miRNA: 3'- uacAUGGCGACGGUGUag-UGCGc--CGc -5' |
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| 10424 | 3' | -57.5 | NC_002687.1 | + | 66863 | 0.66 | 0.947074 |
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Target: 5'- -cGUGaaGCgGCCACugaucaCACGUGGCGu -3' miRNA: 3'- uaCAUggCGaCGGUGua----GUGCGCCGC- -5' |
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| 10424 | 3' | -57.5 | NC_002687.1 | + | 329467 | 0.66 | 0.947074 |
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Target: 5'- gAUGUaagAUCGCUGCCACugcGUCACa-GGUGg -3' miRNA: 3'- -UACA---UGGCGACGGUG---UAGUGcgCCGC- -5' |
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| 10424 | 3' | -57.5 | NC_002687.1 | + | 226802 | 0.66 | 0.95108 |
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Target: 5'- -gGUGCCGgUGCCG-GUgGCGgUGGCGg -3' miRNA: 3'- uaCAUGGCgACGGUgUAgUGC-GCCGC- -5' |
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| 10424 | 3' | -57.5 | NC_002687.1 | + | 34002 | 0.66 | 0.95108 |
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Target: 5'- cGUGUGCCaGCUGUCucuUC-CGCGcGCGa -3' miRNA: 3'- -UACAUGG-CGACGGuguAGuGCGC-CGC- -5' |
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| 10424 | 3' | -57.5 | NC_002687.1 | + | 108106 | 0.66 | 0.958471 |
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Target: 5'- ---cGCgGCgUGCCAaGUC-CGCGGCGg -3' miRNA: 3'- uacaUGgCG-ACGGUgUAGuGCGCCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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