Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 10582 | 5' | -55.5 | NC_002687.1 | + | 253798 | 0.67 | 0.970059 |
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Target: 5'- aUC-CugGCGCUGGCCcgaACCCUGuuggGCGa -3' miRNA: 3'- cAGcGugUGCGACUGG---UGGGACu---UGU- -5' |
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| 10582 | 5' | -55.5 | NC_002687.1 | + | 13618 | 0.67 | 0.9753 |
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Target: 5'- -aCGCACACGUUucccguugugucGAUuucgcuaacacuUACCCUGAACAc -3' miRNA: 3'- caGCGUGUGCGA------------CUG------------GUGGGACUUGU- -5' |
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| 10582 | 5' | -55.5 | NC_002687.1 | + | 300635 | 0.66 | 0.977653 |
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Target: 5'- uGUCGCGCuCGCUucaaucguaGCCACCC-GAGCc -3' miRNA: 3'- -CAGCGUGuGCGAc--------UGGUGGGaCUUGu -5' |
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| 10582 | 5' | -55.5 | NC_002687.1 | + | 80458 | 0.66 | 0.981858 |
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Target: 5'- -aCGCGCACG-UGACCACCggCUcGAugAa -3' miRNA: 3'- caGCGUGUGCgACUGGUGG--GA-CUugU- -5' |
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| 10582 | 5' | -55.5 | NC_002687.1 | + | 257696 | 0.66 | 0.983723 |
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Target: 5'- aUCGCG-GCGCUGuCaGCCUUGAACGc -3' miRNA: 3'- cAGCGUgUGCGACuGgUGGGACUUGU- -5' |
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| 10582 | 5' | -55.5 | NC_002687.1 | + | 107925 | 0.66 | 0.983723 |
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Target: 5'- uUCGCaacGCACGCcaccgagaGCCACCUUGAugGg -3' miRNA: 3'- cAGCG---UGUGCGac------UGGUGGGACUugU- -5' |
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| 10582 | 5' | -55.5 | NC_002687.1 | + | 240478 | 0.66 | 0.983723 |
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Target: 5'- uUUGCAUGCGCUGcacggaucACCGaucuUCCUGAGCu -3' miRNA: 3'- cAGCGUGUGCGAC--------UGGU----GGGACUUGu -5' |
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| 10582 | 5' | -55.5 | NC_002687.1 | + | 220079 | 0.66 | 0.983723 |
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Target: 5'- -cCGUaACGCGCUGACCuCCgUGGcCAa -3' miRNA: 3'- caGCG-UGUGCGACUGGuGGgACUuGU- -5' |
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| 10582 | 5' | -55.5 | NC_002687.1 | + | 143024 | 0.66 | 0.985439 |
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Target: 5'- uGUUGCGCACGUcGAUgaaGCCCUcggGGGCAu -3' miRNA: 3'- -CAGCGUGUGCGaCUGg--UGGGA---CUUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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