miRNA: 3'- guaCCaCAGCCU-GUAUGCGAGa-CgCG- -5'
miRNA: 3'- gUACCACAGCCUguauGC-GAGACGCg -5'
miRNA: 3'- guACCaCAGC-CUGUaugCGAGACgCG- -5'
miRNA: 3'- guaccacAGCCUGUAUGCGAGa--CGCg -5'
miRNA: 3'- -GUACCACaGCCugUAUG-------CGaGACGCg -5'
Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 10597 | 3' | -53.2 | NC_002687.1 | + | 299828 | 0.66 | 0.9962 |
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Target: 5'- --aGGaGUCGGGcCAUGCGCUCgaGgGUu -3' miRNA: 3'- guaCCaCAGCCU-GUAUGCGAGa-CgCG- -5' |
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| 10597 | 3' | -53.2 | NC_002687.1 | + | 241691 | 0.66 | 0.996736 |
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Target: 5'- gGUGGUGUCGGGuacuCGuUUCUGUGg -3' miRNA: 3'- gUACCACAGCCUguauGC-GAGACGCg -5' |
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| 10597 | 3' | -53.2 | NC_002687.1 | + | 64576 | 0.66 | 0.997208 |
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Target: 5'- -uUGGgGUCGaGGCAa--GCUCUGgGCu -3' miRNA: 3'- guACCaCAGC-CUGUaugCGAGACgCG- -5' |
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| 10597 | 3' | -53.2 | NC_002687.1 | + | 317199 | 0.66 | 0.997208 |
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Target: 5'- ------aUCGGGCAUAUGCUCgagGCGg -3' miRNA: 3'- guaccacAGCCUGUAUGCGAGa--CGCg -5' |
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| 10597 | 3' | -53.2 | NC_002687.1 | + | 209992 | 0.66 | 0.997582 |
|
Target: 5'- cCGUGGUGcCGGugGUGCagaugguGC-CUGUGg -3' miRNA: 3'- -GUACCACaGCCugUAUG-------CGaGACGCg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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