Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 10625 | 3' | -65.6 | NC_002702.1 | + | 11146 | 0.67 | 0.136159 |
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Target: 5'- -uCCUCuC-CGCCACGGGG-GCGUUCa -3' miRNA: 3'- gcGGAGcGuGCGGUGCCCCgCGCGGG- -5' |
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| 10625 | 3' | -65.6 | NC_002702.1 | + | 10451 | 0.71 | 0.078926 |
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Target: 5'- -uCCUCaGCGcCGCCGucgcCGGGGCGC-CCCa -3' miRNA: 3'- gcGGAG-CGU-GCGGU----GCCCCGCGcGGG- -5' |
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| 10625 | 3' | -65.6 | NC_002702.1 | + | 13662 | 0.71 | 0.072427 |
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Target: 5'- gGUCUgGguUGCCGCGGcauguacGGCgGCGCCCg -3' miRNA: 3'- gCGGAgCguGCGGUGCC-------CCG-CGCGGG- -5' |
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| 10625 | 3' | -65.6 | NC_002702.1 | + | 13974 | 0.71 | 0.068511 |
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Target: 5'- uCGCCUCGCuCGCCGCagcaGGGCucuggccGCGCgCa -3' miRNA: 3'- -GCGGAGCGuGCGGUGc---CCCG-------CGCGgG- -5' |
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| 10625 | 3' | -65.6 | NC_002702.1 | + | 23994 | 0.73 | 0.055563 |
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Target: 5'- gGCC-CGCACGCUACcuguaccagcccauGGGUGCGCCg -3' miRNA: 3'- gCGGaGCGUGCGGUGc-------------CCCGCGCGGg -5' |
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| 10625 | 3' | -65.6 | NC_002702.1 | + | 14681 | 0.74 | 0.042656 |
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Target: 5'- aCGCCUCGcCAgGCCGCuaagaGGGGUgccaGCGCCg -3' miRNA: 3'- -GCGGAGC-GUgCGGUG-----CCCCG----CGCGGg -5' |
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| 10625 | 3' | -65.6 | NC_002702.1 | + | 32455 | 1.11 | 4.1e-05 |
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Target: 5'- cCGCCUCGCACGCCACGGGGCGCGCCCg -3' miRNA: 3'- -GCGGAGCGUGCGGUGCCCCGCGCGGG- -5' |
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| 10625 | 3' | -65.6 | NC_002702.1 | + | 167 | 1.11 | 4.1e-05 |
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Target: 5'- cCGCCUCGCACGCCACGGGGCGCGCCCg -3' miRNA: 3'- -GCGGAGCGUGCGGUGCCCCGCGCGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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