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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 10628 | 5' | -56.9 | NC_002702.1 | + | 29389 | 0.66 | 0.408867 |
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Target: 5'- uGAGUGGAGc--GAGGAuguaauuuaucuGGAGCCcgGAGa -3' miRNA: 3'- -UUCACCUCuacCUCCU------------CCUCGGa-CUC- -5' |
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| 10628 | 5' | -56.9 | NC_002702.1 | + | 28321 | 0.68 | 0.319222 |
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Target: 5'- gAAGUGGAcuGggGGAGGGGGAcuaacuGCCaGGGu -3' miRNA: 3'- -UUCACCU--CuaCCUCCUCCU------CGGaCUC- -5' |
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| 10628 | 5' | -56.9 | NC_002702.1 | + | 19018 | 0.66 | 0.408867 |
|
Target: 5'- gAGGUGGA--UGcGAuGGAGGAGCCUa-- -3' miRNA: 3'- -UUCACCUcuAC-CU-CCUCCUCGGAcuc -5' |
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| 10628 | 5' | -56.9 | NC_002702.1 | + | 15283 | 0.72 | 0.185931 |
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Target: 5'- -cGUGGcAGAcgacggggucUGGuGGGcGGAGCCUGAGg -3' miRNA: 3'- uuCACC-UCU----------ACCuCCU-CCUCGGACUC- -5' |
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| 10628 | 5' | -56.9 | NC_002702.1 | + | 9576 | 0.69 | 0.287732 |
|
Target: 5'- gGAGcgGGAGggGGAGGAGGGGgagGGGg -3' miRNA: 3'- -UUCa-CCUCuaCCUCCUCCUCggaCUC- -5' |
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| 10628 | 5' | -56.9 | NC_002702.1 | + | 8312 | 1.07 | 0.000371 |
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Target: 5'- gAAGUGGAGAUGGAGGAGGAGCCUGAGg -3' miRNA: 3'- -UUCACCUCUACCUCCUCCUCGGACUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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