Results 1 - 7 of 7 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
10637 | 5' | -61.3 | NC_002702.1 | + | 14119 | 0.66 | 0.298335 |
Target: 5'- uGGCGcugcGACGAgGGGCGGagacgaccaccgcCggcGCGGCGg -3' miRNA: 3'- -CCGCa---CUGCUgCCCGUC-------------Ga--CGCCGCa -5' |
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10637 | 5' | -61.3 | NC_002702.1 | + | 10424 | 0.66 | 0.29173 |
Target: 5'- uGGcCG-GACGACGaGGCcggaacgggguaGGCUGCgcaGGCGg -3' miRNA: 3'- -CC-GCaCUGCUGC-CCG------------UCGACG---CCGCa -5' |
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10637 | 5' | -61.3 | NC_002702.1 | + | 4438 | 0.66 | 0.270537 |
Target: 5'- aGCGUGGCuucuguGACGGuaaaCGGC-GCGGCGUu -3' miRNA: 3'- cCGCACUG------CUGCCc---GUCGaCGCCGCA- -5' |
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10637 | 5' | -61.3 | NC_002702.1 | + | 13902 | 0.67 | 0.257109 |
Target: 5'- aGCGUG-CG-CGcGGCcagagcccuGCUGCGGCGa -3' miRNA: 3'- cCGCACuGCuGC-CCGu--------CGACGCCGCa -5' |
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10637 | 5' | -61.3 | NC_002702.1 | + | 10125 | 0.68 | 0.225924 |
Target: 5'- aGCGUaAUGACGGaGUAGUUGaUGGCGg -3' miRNA: 3'- cCGCAcUGCUGCC-CGUCGAC-GCCGCa -5' |
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10637 | 5' | -61.3 | NC_002702.1 | + | 7370 | 0.69 | 0.177883 |
Target: 5'- -aCGUGGaGAUGGGgGGCaGCGGCGg -3' miRNA: 3'- ccGCACUgCUGCCCgUCGaCGCCGCa -5' |
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10637 | 5' | -61.3 | NC_002702.1 | + | 14717 | 1.08 | 0.000154 |
Target: 5'- aGGCGUGACGACGGGCAGCUGCGGCGUc -3' miRNA: 3'- -CCGCACUGCUGCCCGUCGACGCCGCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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