Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 10638 | 5' | -52 | NC_002702.1 | + | 23165 | 0.66 | 0.751737 |
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Target: 5'- -aGUUGGAGgaGGCGgCGGCAGAAu- -3' miRNA: 3'- acUAGCCUUggUCGCgGCCGUUUUuc -5' |
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| 10638 | 5' | -52 | NC_002702.1 | + | 14105 | 0.66 | 0.729365 |
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Target: 5'- gGggCGGAgacgACCAcCGCCGGCGc---- -3' miRNA: 3'- aCuaGCCU----UGGUcGCGGCCGUuuuuc -5' |
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| 10638 | 5' | -52 | NC_002702.1 | + | 5786 | 0.66 | 0.706551 |
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Target: 5'- -cAUCGGAugCgucaaGGCGCUGGCGu---- -3' miRNA: 3'- acUAGCCUugG-----UCGCGGCCGUuuuuc -5' |
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| 10638 | 5' | -52 | NC_002702.1 | + | 7376 | 0.67 | 0.683403 |
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Target: 5'- aGAUgGGGGgCAGCGgCGGUAGu--- -3' miRNA: 3'- aCUAgCCUUgGUCGCgGCCGUUuuuc -5' |
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| 10638 | 5' | -52 | NC_002702.1 | + | 16005 | 0.67 | 0.683403 |
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Target: 5'- cGAcCGGuGCCAgauGCGCUGGUGGAAGa -3' miRNA: 3'- aCUaGCCuUGGU---CGCGGCCGUUUUUc -5' |
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| 10638 | 5' | -52 | NC_002702.1 | + | 14244 | 0.67 | 0.664719 |
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Target: 5'- aGAUCGuGGcgccagagaucuaugGCCGGCGCCGGa--GGGGu -3' miRNA: 3'- aCUAGC-CU---------------UGGUCGCGGCCguuUUUC- -5' |
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| 10638 | 5' | -52 | NC_002702.1 | + | 15499 | 0.67 | 0.660032 |
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Target: 5'- aUGAgCGGAggcuuuuuGCCGGCGCUGGUu----- -3' miRNA: 3'- -ACUaGCCU--------UGGUCGCGGCCGuuuuuc -5' |
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| 10638 | 5' | -52 | NC_002702.1 | + | 18846 | 0.68 | 0.601301 |
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Target: 5'- --cUCGGGGaagagCGGCGCUGGCAAAcccuGAGa -3' miRNA: 3'- acuAGCCUUg----GUCGCGGCCGUUU----UUC- -5' |
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| 10638 | 5' | -52 | NC_002702.1 | + | 11286 | 0.69 | 0.543301 |
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Target: 5'- cGAaCGuGAGCC-GCGCCcuaGGCGGGGAGg -3' miRNA: 3'- aCUaGC-CUUGGuCGCGG---CCGUUUUUC- -5' |
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| 10638 | 5' | -52 | NC_002702.1 | + | 15568 | 1.09 | 0.001082 |
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Target: 5'- aUGAUCGGAACCAGCGCCGGCAAAAAGc -3' miRNA: 3'- -ACUAGCCUUGGUCGCGGCCGUUUUUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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