Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
10642 | 5' | -49.3 | NC_002702.1 | + | 22817 | 0.71 | 0.642365 |
Target: 5'- cGCUCGUCca-GGCG-CCGGCCGuGAGUu -3' miRNA: 3'- -CGAGCAGuagCUGCuGGUUGGU-UUCG- -5' |
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10642 | 5' | -49.3 | NC_002702.1 | + | 21080 | 1.14 | 0.001155 |
Target: 5'- gGCUCGUCAUCGACGACCAACCAAAGCc -3' miRNA: 3'- -CGAGCAGUAGCUGCUGGUUGGUUUCG- -5' |
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10642 | 5' | -49.3 | NC_002702.1 | + | 21027 | 0.8 | 0.228443 |
Target: 5'- gGUUgGUCGUCGAUGACgAGCCuGGGCu -3' miRNA: 3'- -CGAgCAGUAGCUGCUGgUUGGuUUCG- -5' |
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10642 | 5' | -49.3 | NC_002702.1 | + | 7297 | 0.68 | 0.829136 |
Target: 5'- aCUCGUCcUCuguuguaGACGACCA--CAGAGCc -3' miRNA: 3'- cGAGCAGuAG-------CUGCUGGUugGUUUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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