Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 10649 | 5' | -64.7 | NC_002702.1 | + | 14251 | 0.66 | 0.193527 |
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Target: 5'- gGCGCCAgagaucuaUGGCCg-GcGCCGGAGG-GGu -3' miRNA: 3'- -UGCGGUa-------GCCGGgaC-CGGCCUCCgCC- -5' |
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| 10649 | 5' | -64.7 | NC_002702.1 | + | 15995 | 0.69 | 0.113268 |
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Target: 5'- uCGCCAgauccaCGGCgCCgUGGauaCCGGAGGCGa -3' miRNA: 3'- uGCGGUa-----GCCG-GG-ACC---GGCCUCCGCc -5' |
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| 10649 | 5' | -64.7 | NC_002702.1 | + | 14922 | 0.71 | 0.08107 |
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Target: 5'- uGCGCCGUcugggccuagcaaCGGCCCUGGCgGGcaaaagaaAGGCc- -3' miRNA: 3'- -UGCGGUA-------------GCCGGGACCGgCC--------UCCGcc -5' |
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| 10649 | 5' | -64.7 | NC_002702.1 | + | 12694 | 0.73 | 0.059716 |
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Target: 5'- aAUGCCAUcaCGGagcacuaUCUGGCCGuGGGGCGGc -3' miRNA: 3'- -UGCGGUA--GCCg------GGACCGGC-CUCCGCC- -5' |
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| 10649 | 5' | -64.7 | NC_002702.1 | + | 13821 | 1.08 | 0.000081 |
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Target: 5'- aACGCCAUCGGCCCUGGCCGGAGGCGGc -3' miRNA: 3'- -UGCGGUAGCCGGGACCGGCCUCCGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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