Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 10653 | 3' | -57 | NC_002702.1 | + | 16588 | 0.66 | 0.431318 |
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Target: 5'- cCCAGCUUaaaguaggUCUCUGUGGCCUggGCGAa -3' miRNA: 3'- cGGUCGAAga------AGGGGUACCGGG--UGUU- -5' |
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| 10653 | 3' | -57 | NC_002702.1 | + | 25168 | 0.66 | 0.392182 |
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Target: 5'- uGUCGGC-UCUUCCaguugCCAUGGCuagcaCCACAu -3' miRNA: 3'- -CGGUCGaAGAAGG-----GGUACCG-----GGUGUu -5' |
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| 10653 | 3' | -57 | NC_002702.1 | + | 102 | 0.67 | 0.3735 |
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Target: 5'- gGCgGGCgggCgcgCCCCGUGGCgUGCGAg -3' miRNA: 3'- -CGgUCGaa-Gaa-GGGGUACCGgGUGUU- -5' |
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| 10653 | 3' | -57 | NC_002702.1 | + | 32520 | 0.67 | 0.3735 |
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Target: 5'- gGCgGGCgggCgcgCCCCGUGGCgUGCGAg -3' miRNA: 3'- -CGgUCGaa-Gaa-GGGGUACCGgGUGUU- -5' |
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| 10653 | 3' | -57 | NC_002702.1 | + | 18793 | 0.68 | 0.321173 |
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Target: 5'- uGCCAGCgccgcUCUUCCCCGagaaGGCCa---- -3' miRNA: 3'- -CGGUCGa----AGAAGGGGUa---CCGGguguu -5' |
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| 10653 | 3' | -57 | NC_002702.1 | + | 18148 | 1.08 | 0.000304 |
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Target: 5'- cGCCAGCUUCUUCCCCAUGGCCCACAAc -3' miRNA: 3'- -CGGUCGAAGAAGGGGUACCGGGUGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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