Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
10658 | 3' | -57.2 | NC_002702.1 | + | 12593 | 0.66 | 0.480234 |
Target: 5'- aGCGGuGACGUUCGGcauGUUGGUGUgaaGGAu -3' miRNA: 3'- gUGCCuCUGCGGGUC---CAAUCACG---CCU- -5' |
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10658 | 3' | -57.2 | NC_002702.1 | + | 18333 | 0.66 | 0.459605 |
Target: 5'- aAUGGAGAUGggCAGGUUAGaagccccgGCGGGg -3' miRNA: 3'- gUGCCUCUGCggGUCCAAUCa-------CGCCU- -5' |
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10658 | 3' | -57.2 | NC_002702.1 | + | 16286 | 0.74 | 0.134012 |
Target: 5'- cCACGGGGGCGgCgGGGggcaccgcugugGGUGCGGAa -3' miRNA: 3'- -GUGCCUCUGCgGgUCCaa----------UCACGCCU- -5' |
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10658 | 3' | -57.2 | NC_002702.1 | + | 23955 | 1.09 | 0.000347 |
Target: 5'- cCACGGAGACGCCCAGGUUAGUGCGGAa -3' miRNA: 3'- -GUGCCUCUGCGGGUCCAAUCACGCCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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