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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 10659 | 5' | -54 | NC_002702.1 | + | 15281 | 0.68 | 0.519146 |
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Target: 5'- uGGCAgacgacggGGUCUGGuGgGCGGAGCcugagGCg -3' miRNA: 3'- -UCGU--------UCAGGCCuUgCGCCUCGaua--CG- -5' |
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| 10659 | 5' | -54 | NC_002702.1 | + | 32265 | 0.68 | 0.508118 |
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Target: 5'- cGGCGAGUCuCGaaucGAAUGUGGAGagaaCUGUGUc -3' miRNA: 3'- -UCGUUCAG-GC----CUUGCGCCUC----GAUACG- -5' |
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| 10659 | 5' | -54 | NC_002702.1 | + | 8003 | 0.68 | 0.497191 |
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Target: 5'- cGCAGGUUguggUGGAgauaauucaGCGUGGuGGCUAUGUg -3' miRNA: 3'- uCGUUCAG----GCCU---------UGCGCC-UCGAUACG- -5' |
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| 10659 | 5' | -54 | NC_002702.1 | + | 10416 | 0.69 | 0.434072 |
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Target: 5'- cGaCGAGgCCGGAACGgGGuaGGCUGcGCa -3' miRNA: 3'- uC-GUUCaGGCCUUGCgCC--UCGAUaCG- -5' |
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| 10659 | 5' | -54 | NC_002702.1 | + | 32029 | 1.13 | 0.000351 |
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Target: 5'- cAGCAAGUCCGGAACGCGGAGCUAUGCg -3' miRNA: 3'- -UCGUUCAGGCCUUGCGCCUCGAUACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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