Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1067 | 3' | -55 | NC_000929.1 | + | 27766 | 0.65 | 0.657826 |
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Target: 5'- gGGGUGAa--CGUGCCaccgGGCACGg -3' miRNA: 3'- gCCUAUUuagGCACGGgccaCCGUGC- -5' |
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| 1067 | 3' | -55 | NC_000929.1 | + | 12800 | 0.66 | 0.646566 |
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Target: 5'- gCGGAUuucuuUCUc-GCgCCGGUGGCugGa -3' miRNA: 3'- -GCCUAuuu--AGGcaCG-GGCCACCGugC- -5' |
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| 1067 | 3' | -55 | NC_000929.1 | + | 15305 | 0.67 | 0.556853 |
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Target: 5'- uGGAUAucgggcgaCGUG-CCGGUGGCAUu -3' miRNA: 3'- gCCUAUuuag----GCACgGGCCACCGUGc -5' |
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| 1067 | 3' | -55 | NC_000929.1 | + | 14970 | 0.68 | 0.524003 |
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Target: 5'- uGGGaAAAUgCgCGUG-CCGGUGGCGCu -3' miRNA: 3'- gCCUaUUUA-G-GCACgGGCCACCGUGc -5' |
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| 1067 | 3' | -55 | NC_000929.1 | + | 14176 | 0.68 | 0.502518 |
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Target: 5'- -aGAUAAccgCCGUuaCCGGUGGCAUc -3' miRNA: 3'- gcCUAUUua-GGCAcgGGCCACCGUGc -5' |
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| 1067 | 3' | -55 | NC_000929.1 | + | 27835 | 1.09 | 0.000727 |
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Target: 5'- cCGGAUAAAUCCGUGCCCGGUGGCACGu -3' miRNA: 3'- -GCCUAUUUAGGCACGGGCCACCGUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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