Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 107 | 3' | -58.4 | AC_000006.1 | + | 14134 | 0.66 | 0.411834 |
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Target: 5'- --gCUGGGACCCU-GUGaCCAAGCUGg -3' miRNA: 3'- aggGGCUCUGGGGuCACgGGUUUGAC- -5' |
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| 107 | 3' | -58.4 | AC_000006.1 | + | 8065 | 0.66 | 0.384012 |
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Target: 5'- gUCCUCGAGgaGCCCCAGggugaGuCCCAcGCa- -3' miRNA: 3'- -AGGGGCUC--UGGGGUCa----C-GGGUuUGac -5' |
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| 107 | 3' | -58.4 | AC_000006.1 | + | 3788 | 0.67 | 0.375019 |
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Target: 5'- gCCCU--GACCCagcaGGUGUCCGAGCUc -3' miRNA: 3'- aGGGGcuCUGGGg---UCACGGGUUUGAc -5' |
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| 107 | 3' | -58.4 | AC_000006.1 | + | 23151 | 0.68 | 0.324117 |
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Target: 5'- gUCCCCGAGGCCCU--UGCCa------ -3' miRNA: 3'- -AGGGGCUCUGGGGucACGGguuugac -5' |
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| 107 | 3' | -58.4 | AC_000006.1 | + | 4593 | 0.68 | 0.309107 |
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Target: 5'- gCCCCGGGGCCCgAGggaaaccaucguccCCCAAuCUGg -3' miRNA: 3'- aGGGGCUCUGGGgUCac------------GGGUUuGAC- -5' |
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| 107 | 3' | -58.4 | AC_000006.1 | + | 22423 | 0.68 | 0.293141 |
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Target: 5'- -gCCCGnGAcCCCCGGUGUCCuGAACg- -3' miRNA: 3'- agGGGCuCU-GGGGUCACGGG-UUUGac -5' |
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| 107 | 3' | -58.4 | AC_000006.1 | + | 5140 | 0.7 | 0.24394 |
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Target: 5'- aCCCUGAGACCCUGGaaggacaugacgcUGgCCGcGCUGg -3' miRNA: 3'- aGGGGCUCUGGGGUC-------------ACgGGUuUGAC- -5' |
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| 107 | 3' | -58.4 | AC_000006.1 | + | 1595 | 0.71 | 0.202895 |
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Target: 5'- cUCCCUGAaggucuguGCUCCAGUGCCCAggUg- -3' miRNA: 3'- -AGGGGCUc-------UGGGGUCACGGGUuuGac -5' |
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| 107 | 3' | -58.4 | AC_000006.1 | + | 17601 | 0.72 | 0.167515 |
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Target: 5'- gUCCCCGcccGCCgCCAG-GCCCAGaACUGg -3' miRNA: 3'- -AGGGGCuc-UGG-GGUCaCGGGUU-UGAC- -5' |
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| 107 | 3' | -58.4 | AC_000006.1 | + | 21434 | 1.09 | 0.000252 |
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Target: 5'- uUCCCCGAGACCCCAGUGCCCAAACUGg -3' miRNA: 3'- -AGGGGCUCUGGGGUCACGGGUUUGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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