Results 1 - 19 of 19 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
1071 | 3' | -48.7 | NC_000929.1 | + | 32102 | 0.66 | 0.922617 |
Target: 5'- --aCAUGUGGUuugaAACAgGCgACAACgGCa -3' miRNA: 3'- aaaGUGCACUA----UUGU-CGgUGUUGgCG- -5' |
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1071 | 3' | -48.7 | NC_000929.1 | + | 22999 | 0.66 | 0.909043 |
Target: 5'- -aUCACG-GAUGGCGucaCCACGGCCu- -3' miRNA: 3'- aaAGUGCaCUAUUGUc--GGUGUUGGcg -5' |
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1071 | 3' | -48.7 | NC_000929.1 | + | 11632 | 0.66 | 0.909043 |
Target: 5'- ----uCGUGAgcuGCGGCCugAugUGCu -3' miRNA: 3'- aaaguGCACUau-UGUCGGugUugGCG- -5' |
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1071 | 3' | -48.7 | NC_000929.1 | + | 34895 | 0.66 | 0.909043 |
Target: 5'- --aUugGUGGcGAUAGUCaggucuggACAACCGCa -3' miRNA: 3'- aaaGugCACUaUUGUCGG--------UGUUGGCG- -5' |
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1071 | 3' | -48.7 | NC_000929.1 | + | 18789 | 0.66 | 0.901768 |
Target: 5'- ---uGCGUGAUGAacuGGCacagGCGACUGCa -3' miRNA: 3'- aaagUGCACUAUUg--UCGg---UGUUGGCG- -5' |
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1071 | 3' | -48.7 | NC_000929.1 | + | 11688 | 0.66 | 0.901768 |
Target: 5'- --aCAUcaGGccGCAGCuCACGACCGCa -3' miRNA: 3'- aaaGUGcaCUauUGUCG-GUGUUGGCG- -5' |
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1071 | 3' | -48.7 | NC_000929.1 | + | 15527 | 0.66 | 0.89725 |
Target: 5'- --aCGCG-GAUGugGGCCAgcuugcccgcaguauUAACCGUg -3' miRNA: 3'- aaaGUGCaCUAUugUCGGU---------------GUUGGCG- -5' |
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1071 | 3' | -48.7 | NC_000929.1 | + | 18723 | 0.66 | 0.894174 |
Target: 5'- aUUUCcaGCGUGuccagcGCGGUCAguGCCGCu -3' miRNA: 3'- -AAAG--UGCACuau---UGUCGGUguUGGCG- -5' |
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1071 | 3' | -48.7 | NC_000929.1 | + | 15071 | 0.67 | 0.869526 |
Target: 5'- -aUCACG-GGcuGgAGCUGCAACCGUu -3' miRNA: 3'- aaAGUGCaCUauUgUCGGUGUUGGCG- -5' |
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1071 | 3' | -48.7 | NC_000929.1 | + | 7554 | 0.67 | 0.859815 |
Target: 5'- gUUUCAcCGUGGUGAauuucccCAGCCACGAauaCGa -3' miRNA: 3'- -AAAGU-GCACUAUU-------GUCGGUGUUg--GCg -5' |
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1071 | 3' | -48.7 | NC_000929.1 | + | 14822 | 0.68 | 0.811585 |
Target: 5'- --gCACGUGAUGcCAGCgCACAggagaaaaaagacGCCGa -3' miRNA: 3'- aaaGUGCACUAUuGUCG-GUGU-------------UGGCg -5' |
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1071 | 3' | -48.7 | NC_000929.1 | + | 7367 | 0.68 | 0.802263 |
Target: 5'- aUUUACGUcaugacgggGAUGACAcCCGCGcaagGCCGCa -3' miRNA: 3'- aAAGUGCA---------CUAUUGUcGGUGU----UGGCG- -5' |
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1071 | 3' | -48.7 | NC_000929.1 | + | 14032 | 0.69 | 0.791705 |
Target: 5'- ---gGCGUGAUGGCaAGCgGCAcaaacACCGUc -3' miRNA: 3'- aaagUGCACUAUUG-UCGgUGU-----UGGCG- -5' |
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1071 | 3' | -48.7 | NC_000929.1 | + | 1728 | 0.69 | 0.791705 |
Target: 5'- gUUCAgcauuuCGUcu--GCAGCCugAACCGCa -3' miRNA: 3'- aAAGU------GCAcuauUGUCGGugUUGGCG- -5' |
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1071 | 3' | -48.7 | NC_000929.1 | + | 29600 | 0.7 | 0.701344 |
Target: 5'- -aUCACGUGAaaa-AGCCAC-GCCGg -3' miRNA: 3'- aaAGUGCACUauugUCGGUGuUGGCg -5' |
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1071 | 3' | -48.7 | NC_000929.1 | + | 29536 | 0.7 | 0.701344 |
Target: 5'- -aUCGcCGUGGaugguggggUGACAGuuaCCGCGACCGCc -3' miRNA: 3'- aaAGU-GCACU---------AUUGUC---GGUGUUGGCG- -5' |
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1071 | 3' | -48.7 | NC_000929.1 | + | 17537 | 0.71 | 0.689534 |
Target: 5'- gUUC-CGUgGAUGACAGCaGC-GCCGCa -3' miRNA: 3'- aAAGuGCA-CUAUUGUCGgUGuUGGCG- -5' |
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1071 | 3' | -48.7 | NC_000929.1 | + | 30135 | 0.72 | 0.581928 |
Target: 5'- ---gAUGUGGUGACAGUCAgcgGGCCGCu -3' miRNA: 3'- aaagUGCACUAUUGUCGGUg--UUGGCG- -5' |
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1071 | 3' | -48.7 | NC_000929.1 | + | 29647 | 1.11 | 0.001772 |
Target: 5'- uUUUCACGUGAUAACAGCCACAACCGCg -3' miRNA: 3'- -AAAGUGCACUAUUGUCGGUGUUGGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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