Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1089 | 3' | -52.6 | NC_000929.1 | + | 9277 | 0.67 | 0.679964 |
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Target: 5'- aCGUGAGCGGCugcgucaggAGGUUuugcaccguGUCGCCgGUGGg -3' miRNA: 3'- cGCACUCGUCG---------UCUAG---------UAGCGG-UACU- -5' |
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| 1089 | 3' | -52.6 | NC_000929.1 | + | 20586 | 0.68 | 0.610375 |
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Target: 5'- aGCGgacGGGCAGcCGGAUgAU-GCCGUGGc -3' miRNA: 3'- -CGCa--CUCGUC-GUCUAgUAgCGGUACU- -5' |
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| 1089 | 3' | -52.6 | NC_000929.1 | + | 20833 | 0.69 | 0.564241 |
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Target: 5'- gGCGUGGaaAGCAGAacggcagCGUCGCCGgaggGAg -3' miRNA: 3'- -CGCACUcgUCGUCUa------GUAGCGGUa---CU- -5' |
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| 1089 | 3' | -52.6 | NC_000929.1 | + | 8667 | 0.71 | 0.448026 |
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Target: 5'- uGCuUGAGUguuucgaggacugucAGCAGcgUGUCGCCAUGAc -3' miRNA: 3'- -CGcACUCG---------------UCGUCuaGUAGCGGUACU- -5' |
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| 1089 | 3' | -52.6 | NC_000929.1 | + | 25287 | 0.72 | 0.369679 |
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Target: 5'- aCGUGGGCAgGCAGGUCugccggucccggaUGCCAUGAc -3' miRNA: 3'- cGCACUCGU-CGUCUAGua-----------GCGGUACU- -5' |
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| 1089 | 3' | -52.6 | NC_000929.1 | + | 24381 | 1.12 | 0.000637 |
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Target: 5'- aGCGUGAGCAGCAGAUCAUCGCCAUGAa -3' miRNA: 3'- -CGCACUCGUCGUCUAGUAGCGGUACU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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