Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 109 | 3' | -65.2 | AC_000006.1 | + | 10060 | 0.67 | 0.14997 |
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Target: 5'- gAGGAccugGCCCCggGCgCGCCGgCCACC-Ca -3' miRNA: 3'- -UCCU----CGGGGagCG-GCGGC-GGUGGaGg -5' |
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| 109 | 3' | -65.2 | AC_000006.1 | + | 10024 | 0.67 | 0.146022 |
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Target: 5'- --uGGCCgCUCuaccgccaGCCGCCGCCGCaCUUCc -3' miRNA: 3'- uccUCGGgGAG--------CGGCGGCGGUG-GAGG- -5' |
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| 109 | 3' | -65.2 | AC_000006.1 | + | 9567 | 0.72 | 0.062346 |
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Target: 5'- uGGucCUCCUCGCCaccauCCGCCGCCUCa -3' miRNA: 3'- uCCucGGGGAGCGGc----GGCGGUGGAGg -5' |
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| 109 | 3' | -65.2 | AC_000006.1 | + | 8900 | 0.72 | 0.067804 |
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Target: 5'- cAGGGGCaUCUCGCUgauGUCGCCgaugGCCUCCa -3' miRNA: 3'- -UCCUCGgGGAGCGG---CGGCGG----UGGAGG- -5' |
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| 109 | 3' | -65.2 | AC_000006.1 | + | 8389 | 0.73 | 0.054181 |
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Target: 5'- cGGGGCCggaaC-CGCCGCUGCCGCCgggagcgcgUCCg -3' miRNA: 3'- uCCUCGGg---GaGCGGCGGCGGUGG---------AGG- -5' |
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| 109 | 3' | -65.2 | AC_000006.1 | + | 7435 | 0.66 | 0.185191 |
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Target: 5'- gAGGAGCUCaaaaaGCUGCCGCaCAUCaaaggCCg -3' miRNA: 3'- -UCCUCGGGgag--CGGCGGCG-GUGGa----GG- -5' |
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| 109 | 3' | -65.2 | AC_000006.1 | + | 6309 | 0.66 | 0.180418 |
|
Target: 5'- -cGAGCCCCUgCGCgGCgaggugGCCACCagCg -3' miRNA: 3'- ucCUCGGGGA-GCGgCGg-----CGGUGGagG- -5' |
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| 109 | 3' | -65.2 | AC_000006.1 | + | 4915 | 0.67 | 0.14997 |
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Target: 5'- -aGAGCUCCUC-UCGCUGggcggggaCCGCCUCCu -3' miRNA: 3'- ucCUCGGGGAGcGGCGGC--------GGUGGAGG- -5' |
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| 109 | 3' | -65.2 | AC_000006.1 | + | 4181 | 0.68 | 0.138409 |
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Target: 5'- aAGGGGCUCCcCGUgGCCacaGUCuCCUCCu -3' miRNA: 3'- -UCCUCGGGGaGCGgCGG---CGGuGGAGG- -5' |
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| 109 | 3' | -65.2 | AC_000006.1 | + | 3762 | 0.67 | 0.166749 |
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Target: 5'- uGGaAGCCCUgaGCCGCCagcugGCCGCC-CUg -3' miRNA: 3'- uCC-UCGGGGagCGGCGG-----CGGUGGaGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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