Results 1 - 15 of 15 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
1093 | 3' | -53.3 | NC_000929.1 | + | 1987 | 0.68 | 0.600352 |
Target: 5'- -cGUGCCacGGcuUUCGCCGGAuuCAGCa- -3' miRNA: 3'- gaCAUGG--CC--AAGUGGCCUuuGUCGgc -5' |
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1093 | 3' | -53.3 | NC_000929.1 | + | 9096 | 0.67 | 0.646004 |
Target: 5'- gUGUACaaaGccaUUCGCCGGAugcGACGGCUGa -3' miRNA: 3'- gACAUGg--Cc--AAGUGGCCU---UUGUCGGC- -5' |
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1093 | 3' | -53.3 | NC_000929.1 | + | 10843 | 0.66 | 0.735869 |
Target: 5'- --aUGCCGGUgauuaCGCCGGAgcgcgugaccaGAUGGCCc -3' miRNA: 3'- gacAUGGCCAa----GUGGCCU-----------UUGUCGGc -5' |
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1093 | 3' | -53.3 | NC_000929.1 | + | 11617 | 0.67 | 0.65627 |
Target: 5'- gUGUGCCGGUUgcggucgugagCugCGGccugaugugcugaGAcaGCAGCCGa -3' miRNA: 3'- gACAUGGCCAA-----------GugGCC-------------UU--UGUCGGC- -5' |
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1093 | 3' | -53.3 | NC_000929.1 | + | 13616 | 0.66 | 0.721584 |
Target: 5'- uCUGUuacgucacgaaucaGCCGuGgUCGCCGGAAGCggaaaaAGCCu -3' miRNA: 3'- -GACA--------------UGGC-CaAGUGGCCUUUG------UCGGc -5' |
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1093 | 3' | -53.3 | NC_000929.1 | + | 13674 | 0.74 | 0.303235 |
Target: 5'- cCUGUACCGGaaugCGCCGaauaaaGAGAguGCCGa -3' miRNA: 3'- -GACAUGGCCaa--GUGGC------CUUUguCGGC- -5' |
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1093 | 3' | -53.3 | NC_000929.1 | + | 15495 | 0.66 | 0.69143 |
Target: 5'- -aGUGCaugaugaGGUUCGCCGGGAAau-CCGu -3' miRNA: 3'- gaCAUGg------CCAAGUGGCCUUUgucGGC- -5' |
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1093 | 3' | -53.3 | NC_000929.1 | + | 18546 | 0.68 | 0.62316 |
Target: 5'- --aUGCCGGUggCACCGGGGucauuggucagcGCAGCa- -3' miRNA: 3'- gacAUGGCCAa-GUGGCCUU------------UGUCGgc -5' |
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1093 | 3' | -53.3 | NC_000929.1 | + | 19909 | 0.74 | 0.273201 |
Target: 5'- uUGUACCGGUcggGCUGGAAAaAGCCGc -3' miRNA: 3'- gACAUGGCCAag-UGGCCUUUgUCGGC- -5' |
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1093 | 3' | -53.3 | NC_000929.1 | + | 21216 | 0.66 | 0.724896 |
Target: 5'- ---gGCCGGUUUACCGGuuccccguuCAGCa- -3' miRNA: 3'- gacaUGGCCAAGUGGCCuuu------GUCGgc -5' |
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1093 | 3' | -53.3 | NC_000929.1 | + | 22532 | 0.68 | 0.604907 |
Target: 5'- cCUGUauggggauuGCCGGUgCACCGGAAccgucauaucuguacGCcGCCa -3' miRNA: 3'- -GACA---------UGGCCAaGUGGCCUU---------------UGuCGGc -5' |
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1093 | 3' | -53.3 | NC_000929.1 | + | 24307 | 0.67 | 0.65741 |
Target: 5'- gCUGUcaGCCGGggCGCUGGguAUcGCUGg -3' miRNA: 3'- -GACA--UGGCCaaGUGGCCuuUGuCGGC- -5' |
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1093 | 3' | -53.3 | NC_000929.1 | + | 25741 | 0.71 | 0.41783 |
Target: 5'- -gGUGCCGGggCGCUGGGcuggauggggaaAGguGCCGg -3' miRNA: 3'- gaCAUGGCCaaGUGGCCU------------UUguCGGC- -5' |
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1093 | 3' | -53.3 | NC_000929.1 | + | 26662 | 0.67 | 0.668791 |
Target: 5'- gCUGUAaCCGGUUCuCUGgGAGAUAcGCUGu -3' miRNA: 3'- -GACAU-GGCCAAGuGGC-CUUUGU-CGGC- -5' |
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1093 | 3' | -53.3 | NC_000929.1 | + | 27554 | 1.11 | 0.000796 |
Target: 5'- aCUGUACCGGUUCACCGGAAACAGCCGg -3' miRNA: 3'- -GACAUGGCCAAGUGGCCUUUGUCGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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