Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 111 | 3' | -56.7 | AC_000006.1 | + | 17517 | 0.71 | 0.242142 |
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Target: 5'- uGGCGCgaugaUGCGCguggUGGGCAUG-CCGGCc -3' miRNA: 3'- gUUGCG-----ACGCGa---ACUCGUGCcGGUCG- -5' |
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| 111 | 3' | -56.7 | AC_000006.1 | + | 16237 | 0.72 | 0.211406 |
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Target: 5'- -cGCGCcgGCGCUUGGGCACcaugaucugcauGGUCGGg -3' miRNA: 3'- guUGCGa-CGCGAACUCGUG------------CCGGUCg -5' |
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| 111 | 3' | -56.7 | AC_000006.1 | + | 14839 | 0.72 | 0.205676 |
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Target: 5'- ---aGCUGCGgaUGAGCugGGaguagaCGGCg -3' miRNA: 3'- guugCGACGCgaACUCGugCCg-----GUCG- -5' |
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| 111 | 3' | -56.7 | AC_000006.1 | + | 20022 | 0.74 | 0.150241 |
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Target: 5'- uCGACGCUGCGCUUGAucucgaacucguucgGCgugaGCagccggucguugccgGGCCAGCu -3' miRNA: 3'- -GUUGCGACGCGAACU---------------CG----UG---------------CCGGUCG- -5' |
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| 111 | 3' | -56.7 | AC_000006.1 | + | 11566 | 0.74 | 0.146851 |
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Target: 5'- -uGCGCcggGCGCUgguaggGGGCGCcGCCGGCg -3' miRNA: 3'- guUGCGa--CGCGAa-----CUCGUGcCGGUCG- -5' |
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| 111 | 3' | -56.7 | AC_000006.1 | + | 5053 | 0.74 | 0.138678 |
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Target: 5'- aGACGaUGgGCguccAGCGCGGCCAGCg -3' miRNA: 3'- gUUGCgACgCGaac-UCGUGCCGGUCG- -5' |
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| 111 | 3' | -56.7 | AC_000006.1 | + | 22910 | 0.79 | 0.066539 |
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Target: 5'- cCGACGCUGgGCUcGAGCAUGGCUAc- -3' miRNA: 3'- -GUUGCGACgCGAaCUCGUGCCGGUcg -5' |
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| 111 | 3' | -56.7 | AC_000006.1 | + | 23477 | 1.1 | 0.000287 |
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Target: 5'- uCAACGCUGCGCUUGAGCACGGCCAGCc -3' miRNA: 3'- -GUUGCGACGCGAACUCGUGCCGGUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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