Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 111 | 3' | -56.7 | AC_000006.1 | + | 20840 | 0.67 | 0.402459 |
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Target: 5'- gUAGCaGgUGCGC--GAGCGCGGguuCCAGCc -3' miRNA: 3'- -GUUG-CgACGCGaaCUCGUGCC---GGUCG- -5' |
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| 111 | 3' | -56.7 | AC_000006.1 | + | 21986 | 0.67 | 0.402459 |
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Target: 5'- gCAGCGC-GCGUUgaagGAGCAac-CCAGCa -3' miRNA: 3'- -GUUGCGaCGCGAa---CUCGUgccGGUCG- -5' |
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| 111 | 3' | -56.7 | AC_000006.1 | + | 15529 | 0.67 | 0.412055 |
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Target: 5'- aGugGCgGCGCggc-GCGCGGCUcGCa -3' miRNA: 3'- gUugCGaCGCGaacuCGUGCCGGuCG- -5' |
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| 111 | 3' | -56.7 | AC_000006.1 | + | 20298 | 0.67 | 0.431663 |
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Target: 5'- gUAGgGCUGCcCUUGGcGCAUGGUgGGUg -3' miRNA: 3'- -GUUgCGACGcGAACU-CGUGCCGgUCG- -5' |
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| 111 | 3' | -56.7 | AC_000006.1 | + | 3734 | 0.67 | 0.44167 |
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Target: 5'- --cUGCUGCuGC-UGGGCAUguagcucgaGGCCAGUc -3' miRNA: 3'- guuGCGACG-CGaACUCGUG---------CCGGUCG- -5' |
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| 111 | 3' | -56.7 | AC_000006.1 | + | 20926 | 0.66 | 0.46207 |
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Target: 5'- aCGGCGCUucgGCGC---AGCAUGGCCucguacucgaAGCu -3' miRNA: 3'- -GUUGCGA---CGCGaacUCGUGCCGG----------UCG- -5' |
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| 111 | 3' | -56.7 | AC_000006.1 | + | 16868 | 0.66 | 0.46207 |
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Target: 5'- aGGCGcCUGCGCaUG-GCcCGccGCCGGCg -3' miRNA: 3'- gUUGC-GACGCGaACuCGuGC--CGGUCG- -5' |
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| 111 | 3' | -56.7 | AC_000006.1 | + | 23477 | 1.1 | 0.000287 |
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Target: 5'- uCAACGCUGCGCUUGAGCACGGCCAGCc -3' miRNA: 3'- -GUUGCGACGCGAACUCGUGCCGGUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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