Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 111 | 3' | -56.7 | AC_000006.1 | + | 20715 | 0.69 | 0.306728 |
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Target: 5'- uGGC-CUGCGCcaUUGucaaCACGGCCGGCc -3' miRNA: 3'- gUUGcGACGCG--AACuc--GUGCCGGUCG- -5' |
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| 111 | 3' | -56.7 | AC_000006.1 | + | 9966 | 0.68 | 0.347962 |
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Target: 5'- gCGGCgGCUG-GCggUGAGC--GGCCAGCg -3' miRNA: 3'- -GUUG-CGACgCGa-ACUCGugCCGGUCG- -5' |
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| 111 | 3' | -56.7 | AC_000006.1 | + | 19362 | 0.68 | 0.351427 |
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Target: 5'- gGGC-CUGCGCUaccgcuccaugcuucUGGGCaACGGCC-GCu -3' miRNA: 3'- gUUGcGACGCGA---------------ACUCG-UGCCGGuCG- -5' |
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| 111 | 3' | -56.7 | AC_000006.1 | + | 6177 | 0.68 | 0.36553 |
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Target: 5'- aCggUGgUGCGCUcgucGGGCACGauccugacgcGCCAGCc -3' miRNA: 3'- -GuuGCgACGCGAa---CUCGUGC----------CGGUCG- -5' |
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| 111 | 3' | -56.7 | AC_000006.1 | + | 11491 | 0.68 | 0.36553 |
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Target: 5'- gCAACGaCcGCauccacaagGCcgUGAGCGCGaGCCGGCg -3' miRNA: 3'- -GUUGC-GaCG---------CGa-ACUCGUGC-CGGUCG- -5' |
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| 111 | 3' | -56.7 | AC_000006.1 | + | 18033 | 0.67 | 0.431663 |
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Target: 5'- aCAACcggGUGCUaGA-CAUGGCCAGCa -3' miRNA: 3'- -GUUGcgaCGCGAaCUcGUGCCGGUCG- -5' |
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| 111 | 3' | -56.7 | AC_000006.1 | + | 2722 | 0.66 | 0.451807 |
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Target: 5'- uUAugGCUGCuGgaUGGGCGUGGUCGGa -3' miRNA: 3'- -GUugCGACG-CgaACUCGUGCCGGUCg -5' |
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| 111 | 3' | -56.7 | AC_000006.1 | + | 22910 | 0.79 | 0.066539 |
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Target: 5'- cCGACGCUGgGCUcGAGCAUGGCUAc- -3' miRNA: 3'- -GUUGCGACgCGAaCUCGUGCCGGUcg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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