Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 111 | 3' | -56.7 | AC_000006.1 | + | 10895 | 0.7 | 0.269338 |
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Target: 5'- ---aGCUGCGCgaGGGCcuggACcGCCAGCg -3' miRNA: 3'- guugCGACGCGaaCUCG----UGcCGGUCG- -5' |
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| 111 | 3' | -56.7 | AC_000006.1 | + | 9966 | 0.68 | 0.347962 |
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Target: 5'- gCGGCgGCUG-GCggUGAGC--GGCCAGCg -3' miRNA: 3'- -GUUG-CGACgCGa-ACUCGugCCGGUCG- -5' |
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| 111 | 3' | -56.7 | AC_000006.1 | + | 8402 | 0.69 | 0.298943 |
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Target: 5'- cCggUGUUGCGCccugaGAGCGCuGGCguGCg -3' miRNA: 3'- -GuuGCGACGCGaa---CUCGUG-CCGguCG- -5' |
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| 111 | 3' | -56.7 | AC_000006.1 | + | 7486 | 0.68 | 0.35667 |
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Target: 5'- gAAUGg-GCGCUcGAgcaGCACGGCCuGCa -3' miRNA: 3'- gUUGCgaCGCGAaCU---CGUGCCGGuCG- -5' |
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| 111 | 3' | -56.7 | AC_000006.1 | + | 6177 | 0.68 | 0.36553 |
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Target: 5'- aCggUGgUGCGCUcgucGGGCACGauccugacgcGCCAGCc -3' miRNA: 3'- -GuuGCgACGCGAa---CUCGUGC----------CGGUCG- -5' |
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| 111 | 3' | -56.7 | AC_000006.1 | + | 5053 | 0.74 | 0.138678 |
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Target: 5'- aGACGaUGgGCguccAGCGCGGCCAGCg -3' miRNA: 3'- gUUGCgACgCGaac-UCGUGCCGGUCG- -5' |
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| 111 | 3' | -56.7 | AC_000006.1 | + | 3734 | 0.67 | 0.44167 |
|
Target: 5'- --cUGCUGCuGC-UGGGCAUguagcucgaGGCCAGUc -3' miRNA: 3'- guuGCGACG-CGaACUCGUG---------CCGGUCG- -5' |
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| 111 | 3' | -56.7 | AC_000006.1 | + | 2722 | 0.66 | 0.451807 |
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Target: 5'- uUAugGCUGCuGgaUGGGCGUGGUCGGa -3' miRNA: 3'- -GUugCGACG-CgaACUCGUGCCGGUCg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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