Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11173 | 5' | -49.2 | NC_002816.1 | + | 38925 | 0.66 | 0.995804 |
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Target: 5'- gUAGCU-GUGACGaaacagguggUCGauGUUGCGCAACAc -3' miRNA: 3'- -GUUGAaCAUUGC----------AGC--CAGCGCGUUGU- -5' |
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| 11173 | 5' | -49.2 | NC_002816.1 | + | 23524 | 0.67 | 0.993329 |
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Target: 5'- cCAACcaaa---GUCGGUCGCGCAGu- -3' miRNA: 3'- -GUUGaacauugCAGCCAGCGCGUUgu -5' |
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| 11173 | 5' | -49.2 | NC_002816.1 | + | 34140 | 0.67 | 0.989801 |
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Target: 5'- aGACaUUGUGA--UCGaauGUCGCGCAACAa -3' miRNA: 3'- gUUG-AACAUUgcAGC---CAGCGCGUUGU- -5' |
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| 11173 | 5' | -49.2 | NC_002816.1 | + | 101137 | 0.68 | 0.984958 |
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Target: 5'- gCGGCgUGaUGGCGagacaUCGGUCGCGCucACAa -3' miRNA: 3'- -GUUGaAC-AUUGC-----AGCCAGCGCGu-UGU- -5' |
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| 11173 | 5' | -49.2 | NC_002816.1 | + | 7612 | 0.68 | 0.980868 |
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Target: 5'- ----gUGUGGCGgugaGGUUGUGCGACGg -3' miRNA: 3'- guugaACAUUGCag--CCAGCGCGUUGU- -5' |
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| 11173 | 5' | -49.2 | NC_002816.1 | + | 30771 | 0.69 | 0.970278 |
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Target: 5'- uCAGCgUGUGuACGUCGGUCGCa----- -3' miRNA: 3'- -GUUGaACAU-UGCAGCCAGCGcguugu -5' |
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| 11173 | 5' | -49.2 | NC_002816.1 | + | 537 | 0.7 | 0.956036 |
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Target: 5'- gAACUUGUAGCaaCGGUUGgGguGCAu -3' miRNA: 3'- gUUGAACAUUGcaGCCAGCgCguUGU- -5' |
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| 11173 | 5' | -49.2 | NC_002816.1 | + | 37334 | 0.71 | 0.942711 |
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Target: 5'- gUggUggGUGugGUCGGU-GUGCAGCAg -3' miRNA: 3'- -GuuGaaCAUugCAGCCAgCGCGUUGU- -5' |
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| 11173 | 5' | -49.2 | NC_002816.1 | + | 120291 | 0.71 | 0.927004 |
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Target: 5'- aCAAUUgc-GACuGUUGGUUGCGCAGCAc -3' miRNA: 3'- -GUUGAacaUUG-CAGCCAGCGCGUUGU- -5' |
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| 11173 | 5' | -49.2 | NC_002816.1 | + | 23867 | 1.09 | 0.010443 |
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Target: 5'- uCAACUUGUAACGUCGGUCGCGCAACAg -3' miRNA: 3'- -GUUGAACAUUGCAGCCAGCGCGUUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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