Results 1 - 15 of 15 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
11174 | 5' | -43.3 | NC_002816.1 | + | 69994 | 0.66 | 0.999999 |
Target: 5'- aACAG-AUCGUCCACCA-GAGUUa--- -3' miRNA: 3'- -UGUUgUAGUAGGUGGUuUUCAAcaac -5' |
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11174 | 5' | -43.3 | NC_002816.1 | + | 4397 | 0.66 | 0.999999 |
Target: 5'- uGCAACAgCAUCuCAUagAAAAGUUGUUu -3' miRNA: 3'- -UGUUGUaGUAG-GUGg-UUUUCAACAAc -5' |
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11174 | 5' | -43.3 | NC_002816.1 | + | 26403 | 0.66 | 0.999998 |
Target: 5'- gGCAGCAUgA-CCACCAGAaaGGUgaUGUa- -3' miRNA: 3'- -UGUUGUAgUaGGUGGUUU--UCA--ACAac -5' |
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11174 | 5' | -43.3 | NC_002816.1 | + | 53053 | 0.66 | 0.999998 |
Target: 5'- uCggUGUCAUCCACCAcAGGUg---- -3' miRNA: 3'- uGuuGUAGUAGGUGGUuUUCAacaac -5' |
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11174 | 5' | -43.3 | NC_002816.1 | + | 75916 | 0.66 | 0.999997 |
Target: 5'- aACAACAUCGUcgcCCACCAGAcacaucaAG-UGUa- -3' miRNA: 3'- -UGUUGUAGUA---GGUGGUUU-------UCaACAac -5' |
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11174 | 5' | -43.3 | NC_002816.1 | + | 457 | 0.67 | 0.999992 |
Target: 5'- cACGACGUgAUCCGCCAAcAAGa----- -3' miRNA: 3'- -UGUUGUAgUAGGUGGUU-UUCaacaac -5' |
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11174 | 5' | -43.3 | NC_002816.1 | + | 62062 | 0.68 | 0.999985 |
Target: 5'- gACAGCGUCGacggUCACCAGGGGUa---- -3' miRNA: 3'- -UGUUGUAGUa---GGUGGUUUUCAacaac -5' |
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11174 | 5' | -43.3 | NC_002816.1 | + | 38494 | 0.68 | 0.99997 |
Target: 5'- aGCggUggCGUUgGCCAAGAGUuUGUUGa -3' miRNA: 3'- -UGuuGuaGUAGgUGGUUUUCA-ACAAC- -5' |
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11174 | 5' | -43.3 | NC_002816.1 | + | 112575 | 0.69 | 0.999897 |
Target: 5'- cACAGCAUUAUCCagcguaguaaACCGAGAGgUGg-- -3' miRNA: 3'- -UGUUGUAGUAGG----------UGGUUUUCaACaac -5' |
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11174 | 5' | -43.3 | NC_002816.1 | + | 25664 | 0.69 | 0.999864 |
Target: 5'- uGCGGCAUCGUCa--CAAGAGggGUUu -3' miRNA: 3'- -UGUUGUAGUAGgugGUUUUCaaCAAc -5' |
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11174 | 5' | -43.3 | NC_002816.1 | + | 115227 | 0.72 | 0.998522 |
Target: 5'- uACAacACAUCGUCCACCuuGucguacaccAGUUGUa- -3' miRNA: 3'- -UGU--UGUAGUAGGUGGuuU---------UCAACAac -5' |
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11174 | 5' | -43.3 | NC_002816.1 | + | 112615 | 0.74 | 0.988686 |
Target: 5'- uGCAACAggcaaUCGUCCGgCA--GGUUGUUGg -3' miRNA: 3'- -UGUUGU-----AGUAGGUgGUuuUCAACAAC- -5' |
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11174 | 5' | -43.3 | NC_002816.1 | + | 11730 | 0.75 | 0.987016 |
Target: 5'- cAUggUGUCGUCCACCGuggcAGAGUUGUc- -3' miRNA: 3'- -UGuuGUAGUAGGUGGU----UUUCAACAac -5' |
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11174 | 5' | -43.3 | NC_002816.1 | + | 44718 | 0.77 | 0.958402 |
Target: 5'- cCGACAUUGUCCGCUAucAGUUGUUu -3' miRNA: 3'- uGUUGUAGUAGGUGGUuuUCAACAAc -5' |
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11174 | 5' | -43.3 | NC_002816.1 | + | 29580 | 1.09 | 0.039497 |
Target: 5'- cACAACAUCAUCCACCAAAAGUUGUUGc -3' miRNA: 3'- -UGUUGUAGUAGGUGGUUUUCAACAAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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