Results 1 - 20 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
11181 | 5' | -50.5 | NC_002816.1 | + | 28733 | 0.66 | 0.987103 |
Target: 5'- uACCUCCAAUAaACCCA-CCGAc--- -3' miRNA: 3'- uUGGAGGUUGUgUGGGUaGGUUaugu -5' |
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11181 | 5' | -50.5 | NC_002816.1 | + | 59755 | 0.66 | 0.985347 |
Target: 5'- cAACCUgcacaCCGAUACACCaAUCUAcgACAa -3' miRNA: 3'- -UUGGA-----GGUUGUGUGGgUAGGUuaUGU- -5' |
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11181 | 5' | -50.5 | NC_002816.1 | + | 60613 | 0.66 | 0.983414 |
Target: 5'- cACCUCCGucCGC-CCCAcccUCCAccucGUACAc -3' miRNA: 3'- uUGGAGGUu-GUGuGGGU---AGGU----UAUGU- -5' |
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11181 | 5' | -50.5 | NC_002816.1 | + | 85128 | 0.66 | 0.981295 |
Target: 5'- cACCguaaUCAACACACCCA-CCAAUc-- -3' miRNA: 3'- uUGGa---GGUUGUGUGGGUaGGUUAugu -5' |
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11181 | 5' | -50.5 | NC_002816.1 | + | 15177 | 0.66 | 0.978979 |
Target: 5'- cAGCCUUCAACAC-CCUAUaCCAGa--- -3' miRNA: 3'- -UUGGAGGUUGUGuGGGUA-GGUUaugu -5' |
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11181 | 5' | -50.5 | NC_002816.1 | + | 32350 | 0.67 | 0.976193 |
Target: 5'- -uUCUCCAACgucauugGCGCCUuuGUCCAAUAUc -3' miRNA: 3'- uuGGAGGUUG-------UGUGGG--UAGGUUAUGu -5' |
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11181 | 5' | -50.5 | NC_002816.1 | + | 60890 | 0.67 | 0.970758 |
Target: 5'- cACCUCCAACaACACCUGcCCucUGCc -3' miRNA: 3'- uUGGAGGUUG-UGUGGGUaGGuuAUGu -5' |
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11181 | 5' | -50.5 | NC_002816.1 | + | 13791 | 0.67 | 0.970758 |
Target: 5'- cACCUUCAAUACACUgGUCaCAAUuuuGCGa -3' miRNA: 3'- uUGGAGGUUGUGUGGgUAG-GUUA---UGU- -5' |
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11181 | 5' | -50.5 | NC_002816.1 | + | 75765 | 0.67 | 0.967566 |
Target: 5'- gAGCCcaCCGACgACGCCCaAUCCAccaaAUGCGa -3' miRNA: 3'- -UUGGa-GGUUG-UGUGGG-UAGGU----UAUGU- -5' |
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11181 | 5' | -50.5 | NC_002816.1 | + | 93481 | 0.67 | 0.967566 |
Target: 5'- cACCUUgAACACAUUUAUCCuguUGCAu -3' miRNA: 3'- uUGGAGgUUGUGUGGGUAGGuu-AUGU- -5' |
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11181 | 5' | -50.5 | NC_002816.1 | + | 106240 | 0.67 | 0.967566 |
Target: 5'- cAGCUUCCAACACauauuguucGCCCAucucUCCAGUuugucggccACAa -3' miRNA: 3'- -UUGGAGGUUGUG---------UGGGU----AGGUUA---------UGU- -5' |
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11181 | 5' | -50.5 | NC_002816.1 | + | 7294 | 0.68 | 0.960455 |
Target: 5'- uGCCgUCCGuACACACCCcacUCCAcaaaGUACGc -3' miRNA: 3'- uUGG-AGGU-UGUGUGGGu--AGGU----UAUGU- -5' |
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11181 | 5' | -50.5 | NC_002816.1 | + | 97602 | 0.68 | 0.956524 |
Target: 5'- cGCUUCCAACACGCUCAUU--GUugAu -3' miRNA: 3'- uUGGAGGUUGUGUGGGUAGguUAugU- -5' |
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11181 | 5' | -50.5 | NC_002816.1 | + | 108600 | 0.68 | 0.947883 |
Target: 5'- -cCCUCCuaauguACACACCCAUaUCAGUGa- -3' miRNA: 3'- uuGGAGGu-----UGUGUGGGUA-GGUUAUgu -5' |
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11181 | 5' | -50.5 | NC_002816.1 | + | 64908 | 0.68 | 0.947883 |
Target: 5'- cAAUUUCuCGACGCGCCCGcCCGcgACAu -3' miRNA: 3'- -UUGGAG-GUUGUGUGGGUaGGUuaUGU- -5' |
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11181 | 5' | -50.5 | NC_002816.1 | + | 65981 | 0.68 | 0.947423 |
Target: 5'- cGCCUCCGACAUguaagucGCCCuacaCCgAAUACAc -3' miRNA: 3'- uUGGAGGUUGUG-------UGGGua--GG-UUAUGU- -5' |
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11181 | 5' | -50.5 | NC_002816.1 | + | 118119 | 0.68 | 0.943164 |
Target: 5'- -cCCUCCAACACaaauuGCUCcgCCAGUAg- -3' miRNA: 3'- uuGGAGGUUGUG-----UGGGuaGGUUAUgu -5' |
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11181 | 5' | -50.5 | NC_002816.1 | + | 54740 | 0.68 | 0.943164 |
Target: 5'- cACacaUUCGACACACaCCGcCCAAUACAu -3' miRNA: 3'- uUGg--AGGUUGUGUG-GGUaGGUUAUGU- -5' |
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11181 | 5' | -50.5 | NC_002816.1 | + | 31412 | 0.69 | 0.932916 |
Target: 5'- cAGCauaCAACACACCCAccUCCAacauGUACAc -3' miRNA: 3'- -UUGgagGUUGUGUGGGU--AGGU----UAUGU- -5' |
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11181 | 5' | -50.5 | NC_002816.1 | + | 112934 | 0.69 | 0.927381 |
Target: 5'- cACCUCCAACACcaacacuacuaACaCCA-CCGAUACc -3' miRNA: 3'- uUGGAGGUUGUG-----------UG-GGUaGGUUAUGu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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