Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11185 | 3' | -52.9 | NC_002816.1 | + | 36996 | 0.66 | 0.964593 |
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Target: 5'- aGugGCCaaACGCCcgGaCgUGGGACACGu -3' miRNA: 3'- aCugCGG--UGUGGaaCaG-ACCUUGUGC- -5' |
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| 11185 | 3' | -52.9 | NC_002816.1 | + | 32276 | 0.66 | 0.949079 |
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Target: 5'- aGAUGCCACAUUUUGUaUGGAu--CGu -3' miRNA: 3'- aCUGCGGUGUGGAACAgACCUuguGC- -5' |
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| 11185 | 3' | -52.9 | NC_002816.1 | + | 103307 | 0.66 | 0.947313 |
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Target: 5'- uUGugGCCAggugcgacaccacCACCcuccaUUGUCUuaucaaucuaaucaGGAACACGa -3' miRNA: 3'- -ACugCGGU-------------GUGG-----AACAGA--------------CCUUGUGC- -5' |
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| 11185 | 3' | -52.9 | NC_002816.1 | + | 59649 | 0.67 | 0.934855 |
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Target: 5'- aGGCGCCACACaCUacuaUGUCUuuuACACu -3' miRNA: 3'- aCUGCGGUGUG-GA----ACAGAccuUGUGc -5' |
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| 11185 | 3' | -52.9 | NC_002816.1 | + | 108183 | 0.68 | 0.912323 |
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Target: 5'- gGugGCaCACACCUcGUUgGuGAGCACa -3' miRNA: 3'- aCugCG-GUGUGGAaCAGaC-CUUGUGc -5' |
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| 11185 | 3' | -52.9 | NC_002816.1 | + | 14597 | 0.68 | 0.906054 |
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Target: 5'- cGACGCCAC-CCUgcacgGUUUGcaAACGCGc -3' miRNA: 3'- aCUGCGGUGuGGAa----CAGACc-UUGUGC- -5' |
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| 11185 | 3' | -52.9 | NC_002816.1 | + | 47147 | 0.68 | 0.88575 |
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Target: 5'- gGACGCCACaACCUcG-CUGaGAaaACGCGu -3' miRNA: 3'- aCUGCGGUG-UGGAaCaGAC-CU--UGUGC- -5' |
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| 11185 | 3' | -52.9 | NC_002816.1 | + | 80651 | 0.69 | 0.846363 |
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Target: 5'- cGACGCCACGCgaaucaaccaagaCUUGUUUGuGGGCGgGu -3' miRNA: 3'- aCUGCGGUGUG-------------GAACAGAC-CUUGUgC- -5' |
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| 11185 | 3' | -52.9 | NC_002816.1 | + | 93259 | 0.73 | 0.66283 |
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Target: 5'- aGACGCCACaaucACCUUGgUUGGGuuGCGCa -3' miRNA: 3'- aCUGCGGUG----UGGAACaGACCU--UGUGc -5' |
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| 11185 | 3' | -52.9 | NC_002816.1 | + | 83771 | 1.1 | 0.003738 |
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Target: 5'- uUGACGCCACACCUUGUCUGGAACACGg -3' miRNA: 3'- -ACUGCGGUGUGGAACAGACCUUGUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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