Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11188 | 5' | -47.7 | NC_002816.1 | + | 12052 | 0.67 | 0.997448 |
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Target: 5'- uUUUCGCUAAAAUUUCuuGaaUAAAGCCa -3' miRNA: 3'- -AAAGCGAUUUUAGAGc-CgcGUUUCGG- -5' |
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| 11188 | 5' | -47.7 | NC_002816.1 | + | 41426 | 0.67 | 0.995711 |
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Target: 5'- -aUCGUU--GAUUUUGGCGgAAAGCg -3' miRNA: 3'- aaAGCGAuuUUAGAGCCGCgUUUCGg -5' |
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| 11188 | 5' | -47.7 | NC_002816.1 | + | 3948 | 0.7 | 0.979869 |
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Target: 5'- ---aGCUccuuguccuccaGAAAUCUUGGCGUGuuGCCa -3' miRNA: 3'- aaagCGA------------UUUUAGAGCCGCGUuuCGG- -5' |
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| 11188 | 5' | -47.7 | NC_002816.1 | + | 98704 | 0.7 | 0.979869 |
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Target: 5'- -gUCGCguacAGAGUcCUCGGCGCcgguGGCa -3' miRNA: 3'- aaAGCGa---UUUUA-GAGCCGCGuu--UCGg -5' |
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| 11188 | 5' | -47.7 | NC_002816.1 | + | 34740 | 0.7 | 0.974669 |
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Target: 5'- -cUUGUUGAAcgacggcuccGUCUUGGCGUucGGCCu -3' miRNA: 3'- aaAGCGAUUU----------UAGAGCCGCGuuUCGG- -5' |
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| 11188 | 5' | -47.7 | NC_002816.1 | + | 48291 | 0.72 | 0.933748 |
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Target: 5'- uUUUCGCUAAAAUUUUaGCcGUAAAGUCa -3' miRNA: 3'- -AAAGCGAUUUUAGAGcCG-CGUUUCGG- -5' |
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| 11188 | 5' | -47.7 | NC_002816.1 | + | 50864 | 0.73 | 0.909668 |
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Target: 5'- -cUCGgcAAAAUCUUGGCGCAAAaCCa -3' miRNA: 3'- aaAGCgaUUUUAGAGCCGCGUUUcGG- -5' |
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| 11188 | 5' | -47.7 | NC_002816.1 | + | 48587 | 0.74 | 0.888665 |
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Target: 5'- -cUCGCcAGAAUCUCGGCaCAAAaCCa -3' miRNA: 3'- aaAGCGaUUUUAGAGCCGcGUUUcGG- -5' |
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| 11188 | 5' | -47.7 | NC_002816.1 | + | 11936 | 1.12 | 0.008507 |
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Target: 5'- uUUUCGCUAAAAUCUCGGCGCAAAGCCa -3' miRNA: 3'- -AAAGCGAUUUUAGAGCCGCGUUUCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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