Results 1 - 14 of 14 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
11189 | 5' | -48.2 | NC_002816.1 | + | 39678 | 0.66 | 0.999111 |
Target: 5'- gGUGCcgguguUGAUGUGGUGACAa-GGGu -3' miRNA: 3'- gUACGuuu---AUUGCACCGCUGUgcUCC- -5' |
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11189 | 5' | -48.2 | NC_002816.1 | + | 18900 | 0.66 | 0.999111 |
Target: 5'- --aGCAAAUGACacaGUGGaUGACACaGuGGa -3' miRNA: 3'- guaCGUUUAUUG---CACC-GCUGUG-CuCC- -5' |
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11189 | 5' | -48.2 | NC_002816.1 | + | 97873 | 0.66 | 0.998908 |
Target: 5'- gGUGCGuGAUGAUauuaUGGCGGugUACGAGGu -3' miRNA: 3'- gUACGU-UUAUUGc---ACCGCU--GUGCUCC- -5' |
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11189 | 5' | -48.2 | NC_002816.1 | + | 107645 | 0.66 | 0.998384 |
Target: 5'- --gGCAAAaguGCGcuguUGGUGGCGCuGAGGa -3' miRNA: 3'- guaCGUUUau-UGC----ACCGCUGUG-CUCC- -5' |
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11189 | 5' | -48.2 | NC_002816.1 | + | 58984 | 0.67 | 0.997062 |
Target: 5'- aUAUGCGGGUAAacgGUGGCGAuuucgcguuugaucCACGAa- -3' miRNA: 3'- -GUACGUUUAUUg--CACCGCU--------------GUGCUcc -5' |
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11189 | 5' | -48.2 | NC_002816.1 | + | 60440 | 0.67 | 0.996688 |
Target: 5'- --gGCAcgagGGUAGgGUGGUG-UACGAGGu -3' miRNA: 3'- guaCGU----UUAUUgCACCGCuGUGCUCC- -5' |
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11189 | 5' | -48.2 | NC_002816.1 | + | 7056 | 0.67 | 0.996688 |
Target: 5'- uGUGCAGA--GCGUGcGCGAguuuCugGAGa -3' miRNA: 3'- gUACGUUUauUGCAC-CGCU----GugCUCc -5' |
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11189 | 5' | -48.2 | NC_002816.1 | + | 31920 | 0.69 | 0.988961 |
Target: 5'- --gGUGGAUGugGUGGUgcgGGCACGGGu -3' miRNA: 3'- guaCGUUUAUugCACCG---CUGUGCUCc -5' |
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11189 | 5' | -48.2 | NC_002816.1 | + | 119022 | 0.7 | 0.974344 |
Target: 5'- aCGUGCAAuggggGUAugGagGGUGGCAaGAGGa -3' miRNA: 3'- -GUACGUU-----UAUugCa-CCGCUGUgCUCC- -5' |
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11189 | 5' | -48.2 | NC_002816.1 | + | 104060 | 0.71 | 0.96138 |
Target: 5'- gGUGCG----GCGUGGCacaaucGACACGAGa -3' miRNA: 3'- gUACGUuuauUGCACCG------CUGUGCUCc -5' |
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11189 | 5' | -48.2 | NC_002816.1 | + | 107127 | 0.72 | 0.934421 |
Target: 5'- uUAUGCGAGggagaugguggAGCGauUGGCG-CGCGAGGg -3' miRNA: 3'- -GUACGUUUa----------UUGC--ACCGCuGUGCUCC- -5' |
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11189 | 5' | -48.2 | NC_002816.1 | + | 40979 | 0.74 | 0.890807 |
Target: 5'- uUAUGUuAAUaGugGUGGUGACgACGAGGa -3' miRNA: 3'- -GUACGuUUA-UugCACCGCUG-UGCUCC- -5' |
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11189 | 5' | -48.2 | NC_002816.1 | + | 105384 | 0.74 | 0.883521 |
Target: 5'- -uUGCAAGUuuAAUuUGGUGugGCGAGGg -3' miRNA: 3'- guACGUUUA--UUGcACCGCugUGCUCC- -5' |
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11189 | 5' | -48.2 | NC_002816.1 | + | 19529 | 1.11 | 0.011012 |
Target: 5'- uCAUGCAAAUAACGUGGCGACACGAGGu -3' miRNA: 3'- -GUACGUUUAUUGCACCGCUGUGCUCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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