Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11191 | 3' | -41.4 | NC_002816.1 | + | 23865 | 0.68 | 1 |
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Target: 5'- aACUg--GGACaAAGUGCGCACUAa--- -3' miRNA: 3'- -UGGaaaCUUG-UUUACGUGUGAUauua -5' |
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| 11191 | 3' | -41.4 | NC_002816.1 | + | 99342 | 0.67 | 1 |
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Target: 5'- uGCUUgaaacaGAGCAAAUGCACAuacuacaacuCUAUGAUu -3' miRNA: 3'- -UGGAaa----CUUGUUUACGUGU----------GAUAUUA- -5' |
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| 11191 | 3' | -41.4 | NC_002816.1 | + | 17573 | 0.66 | 1 |
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Target: 5'- gGCCagUUGggUAAGUGC-CGuCUGUAGg -3' miRNA: 3'- -UGGa-AACuuGUUUACGuGU-GAUAUUa -5' |
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| 11191 | 3' | -41.4 | NC_002816.1 | + | 93694 | 0.66 | 1 |
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Target: 5'- cGCCUUgcgUGAACAAGUGCGagacCUAc--- -3' miRNA: 3'- -UGGAA---ACUUGUUUACGUgu--GAUauua -5' |
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| 11191 | 3' | -41.4 | NC_002816.1 | + | 32881 | 0.68 | 0.999999 |
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Target: 5'- aGCCgaUGAGCGAGUgGCACAUgGUGc- -3' miRNA: 3'- -UGGaaACUUGUUUA-CGUGUGaUAUua -5' |
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| 11191 | 3' | -41.4 | NC_002816.1 | + | 37911 | 0.68 | 0.999999 |
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Target: 5'- cGCCgagaaUGGGCGucaaaAAUGCACACUAUc-- -3' miRNA: 3'- -UGGaa---ACUUGU-----UUACGUGUGAUAuua -5' |
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| 11191 | 3' | -41.4 | NC_002816.1 | + | 54806 | 0.69 | 0.999997 |
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Target: 5'- -aCUUUGAGCGGuUGCGCGCcauguugGUGAUg -3' miRNA: 3'- ugGAAACUUGUUuACGUGUGa------UAUUA- -5' |
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| 11191 | 3' | -41.4 | NC_002816.1 | + | 84699 | 0.72 | 0.999716 |
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Target: 5'- cACCUauuuuagcacGAACAAuAUGCACACUGUGGg -3' miRNA: 3'- -UGGAaa--------CUUGUU-UACGUGUGAUAUUa -5' |
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| 11191 | 3' | -41.4 | NC_002816.1 | + | 26099 | 1.08 | 0.066001 |
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Target: 5'- cACCUUUGAACAAAUGCACACUAUAAUg -3' miRNA: 3'- -UGGAAACUUGUUUACGUGUGAUAUUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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