Results 1 - 8 of 8 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
11191 | 5' | -47.8 | NC_002816.1 | + | 58658 | 0.66 | 0.999374 |
Target: 5'- --cGU-UGGUGUGUUCAUCAggCCGc -3' miRNA: 3'- cguCAcAUCGCACAAGUAGUuaGGCu -5' |
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11191 | 5' | -47.8 | NC_002816.1 | + | 8681 | 0.67 | 0.998261 |
Target: 5'- uCAGUGUGGCGaucgaUGUgag-CGAUCUGAu -3' miRNA: 3'- cGUCACAUCGC-----ACAaguaGUUAGGCU- -5' |
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11191 | 5' | -47.8 | NC_002816.1 | + | 113688 | 0.67 | 0.998261 |
Target: 5'- -aAGUGggAGUGUGUUUGUCAAgCUGGa -3' miRNA: 3'- cgUCACa-UCGCACAAGUAGUUaGGCU- -5' |
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11191 | 5' | -47.8 | NC_002816.1 | + | 121253 | 0.67 | 0.997488 |
Target: 5'- uGguG-GUGGCGUcGagCAUCGAUCCa- -3' miRNA: 3'- -CguCaCAUCGCA-CaaGUAGUUAGGcu -5' |
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11191 | 5' | -47.8 | NC_002816.1 | + | 111563 | 0.68 | 0.994243 |
Target: 5'- cGCGGUGcAGC-UGUUgAUgGAUCUGAa -3' miRNA: 3'- -CGUCACaUCGcACAAgUAgUUAGGCU- -5' |
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11191 | 5' | -47.8 | NC_002816.1 | + | 108646 | 0.7 | 0.984789 |
Target: 5'- -aAGUGUAGCGUG---AUCGGUCCc- -3' miRNA: 3'- cgUCACAUCGCACaagUAGUUAGGcu -5' |
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11191 | 5' | -47.8 | NC_002816.1 | + | 80742 | 0.75 | 0.837337 |
Target: 5'- gGguGUGUGGUGUcGcUCAUCGAUUCGGa -3' miRNA: 3'- -CguCACAUCGCA-CaAGUAGUUAGGCU- -5' |
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11191 | 5' | -47.8 | NC_002816.1 | + | 26134 | 1.13 | 0.008188 |
Target: 5'- cGCAGUGUAGCGUGUUCAUCAAUCCGAg -3' miRNA: 3'- -CGUCACAUCGCACAAGUAGUUAGGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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